PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY
 

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, ISSN No. : 0972-2025, Vol.: 9, Issue.: 3-4

Original Research Article

A Study on Endogenous Level of Kanamycin Resistance of Sesame (Sesamum indicum L.)

 

B.C. Vivek1, R. Lokesha1*, R. Ramachandra1 and P.H. Ramanjini Gowda1

1Plant Tissue culture and Molecular Laboratory, Dept. of Genetics and Plant Breeding, College of Agriculture, Raichur-584 101 (University of Agricultural Sciences, Dharwad) (India) Vaccine Lab, Department of Biotechnology, GKVK, Bangalore-560 0065 (University of Agricultural Sciences, Bangalore) (India)

Abstracts

Investigation was undertaken to study the endogenous level of Kanamycin resistance of Sesamum indicum genotypes under varied concentrations to Kanamycin antibiotic (0 to 150 mg/l). S. indicum genotypes were highly sensitive to Kanamycin exposure; seed germinability decreased with increase in Kanamycin concentration on MS media supplemented with growth regulators (NAA @ 0.5 mg/l, BAP @ 1.5 mg/l and Kinetin @ 1.5mg/l). Seedlings bleached and failed to induce callus irrespective of Kanamycin concentrations whilst the seedlings induced callus and callus proliferated in the absence of Kanamycin. Further, the well-grown callus failed to proliferate on MS with Kanamycin indicating the sensitivity of the callus for proliferation also. The findings are discussed in light of the usage of nptII gene encoding neomycin phospho-transferase, a commonly used selectable marker, which induces Kanamycin resistance for genetic transformation studies in sesame.

Keywords :

Sesamum indicum, Kanamycin, Growth regulators, nptII