PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY
 

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, ISSN No. : 0972-2025, Vol.: 9, Issue.: 3-4

Original Research Article

In vitro Regeneration from Leaf Callus Cultures of Scoparia dulcis

 

M. Jothi Basu1*, A. Kalidoss1, Kayathri1, Shanmugan1 and S. Baburaj1

1Department of Biotechnology, J.J College of Arts and Science, Pudukkottai- 622422, Tamil Nadu, India Department of Botany, Thiagarajar College, Madurai, Tamil Nadu, India Alagappa University, Karaikudi-630 003, Tamil Nadu, India [M.J]

Abstracts

Leaf explants of Scoparia dulcis L differentiated shoot buds from the callus on Murashige and Skoog s (MS) medium containing different combinations and concentrations of auxins and cytokinins or cytokinins alone. Shoot buds were formed either directly from the explants or from the callus. Regenerated shoots were successfully rooted on a medium containing half-strength MS and 2mg/l IAA. The regenerated plants were transferred to green house conditions with 75 to 80% of survival.

Keywords :

Plant regeneration, Leaf callus, Scoparia dulcis, Medicinal herb