PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY
 

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, ISSN No. : 0972-2025, Vol.: 13, Issue.: 1-2

Original Research Article

Effect of NaCl Treatment on In Vitro Shoot Multiplication of Brassica juncea (L.)

 

Indrani Chandra1* and Sutapa Patra1

1Department of Biotechnology, The University of Burdwan, Golapbag, Burdwan-713 104, India.

Abstracts

The present experiment was focused to study the effect of different concentrations of NaCl on shoot multiplication in Brassica juncea. In vitro culture was established on MS medium supplemented with 1.0 mg/l BAP. NaCl at various concentrations was added in the medium to evaluate the effect on shoot growth and multiplication. Among the NaCl concentrations tested, 0.2% was found to be optimum for best shoot multiplication for B. juncea and the highest number of shoots obtained was 6.3�0.19. As the NaCl concentration increased, the morphological abnormalities were also observed. The NaCl at 2.5% level was found to be lethal dose for shoot growth in Brassica.

Keywords :

Culture establishment, NaCl tolerance, BAP, Lethal, Shoot multiplication.