PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY
 

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, ISSN No. : 0972-2025, Vol.: 13, Issue.: 1-2

Original Research Article

Development of an Efficient Protocol for High Frequency Plant Regeneration from Nodal Explants of Solanum nigrum L.

 

D. Mahendran1, S. Srinivasan1, M. Thiyagarajan2 and P. Venkatachalam2

1PG & Research Department of Botany, National College Tiruchirappalli, TN, India [D.M, S.S] 2Plant Genetic Engineering and Molecular Biology Lab, Department of Biotechnology Periyar University Salem-11, TN, India [P.V., M.T.].

Abstracts

An efficient protocol was devised for rapid propagation of Solanum nigrum L. from nodal explants. The nodal explants were cultured on MS medium supplemented with different concentrations (0.5-2.5 mg/l) of BAP and KIN for Multiple shoot bud induction. The highest rate of multiple shoot proliferation was observed on MS medium fortified with BAP (2.0 mg/l) followed by KIN (1.5 mg/l). The adventitious shoots were rooted successively on half strength MS medium supplemented with different concentrations of IBA (0.5-2.5 mg/l). The best rooting response was observed on 1 mg/l IBA. Regenerated rooted plantlets were successfully transferred into plastic cup containing a mixture of soil and sand in the ratio of 1:1. The 90% of the in vitro propagated plantlets survived when they were transferred to ex vitro conditions for acclimatization.

Keywords :

Solanum nigrum L., MS medium, Nodal explants, Shoot multiplication.