Asian Journal of Microbiology and Biotechnology
 

Asian Journal of Microbiology and Biotechnology, ISSN No. : .., Vol.: 1, Issue.: 1

Original Research Article

MEDIA COMPOSITION AFFECTS THE FATTY ACIDS PROFILES OF THREE OLEAGINOUS MICROALGAE FROM THE PERUVIAN AMAZON

 

MARIANELA COBOS1*, LIZ C. LOPEZ1, JAE D. PAREDES1, J. DYLAN MADDOX2,3, GABRIEL E. VARGAS-ARANA1, LEENIN FLORES4, CARLA P. AGUILAR4 AND JUAN C. CASTRO5*

1Laboratorio de Biotecnología y Bioenergética, Universidad Científica del Perú (UCP), Av. Abelardo Quiñones Km 2,5, Iquitos, Postal Code: 6024, Perú.

2The Field Museum of Natural History, Pritzker Laboratory for Molecular Systematics and Evolution, 1400 S. Lake Shore Drive, Chicago, IL, Postal Code: 60605, USA.

3Department of Environmental Sciences, American Public University System, Charles Town, WV, Postal Code: 25414, USA.

4Laboratorio de Biotecnología Acuática, Instituto del Mar del Perú (IMARPE), Calle Esquina General Valle s/n Chucuito-Callao, Postal Code: 07021, Perú.

5Unidad Especializada de Biotecnología, Centro de Investigaciones de Recursos Naturales (CIRNA), Universidad Nacional de la Amazonía Peruana (UNAP), Psje. Los Paujiles S/N, Iquitos, Postal Code: 16024, Perú.

Abstracts

The objective of this research was to determine the effect of four culture media on the fatty acids profile of the microalgae Ankistrodesmus sp., Chlorella sp., and Scenedesmus sp. isolated from the Peruvian Amazon. The microalgae species were growth by triplicate in Beijerinck's medium (BM), Blue Green medium (BG-11), Bold Basal medium (BBM), and CHU-10 medium. Growth was monitored by spectrophotometric analysis at 680 nm. Biomass productivity, biochemical analyzes, and fatty acids profiling were performed using standard methods. The highest growth was obtained with BM, BBM, and CHU-10 medium for the three microalgae species and the biomass productivity was highest (> 0.23 mg.L-1.d-1) in CHU-10 medium. The highest total lipid content was reported with Ankistrodesmus sp. (13.77 - 21.58%). Carbohydrate content varied between 8.46 and 50.17%, with the highest percentage when the microalgae were cultured in BG-11 (27.26 – 37.80%) and CHU-10 media (30.72 – 50.17%). Also, the highest protein content was found in the three microalgae species cultured in BM medium (24.89 - 30.44%). The fatty acids profiles showed differences at the intra-species and inter-species levels. In conclusion, the three microalgae showed differences in their growth profile, biomass productivity, biochemical composition (carbohydrates, total lipids, and proteins contents), and fatty acids profiles. Likewise, these growth and biochemical parameters are markedly affected by the media composition. Additionally, the fatty acids profiles of the three microalgae reveals that are a good feedstocks for biodiesel production. 

Keywords :

Ankistrodesmus; biochemical composition; biodiesel; Chlorella; fatty acids profiling; Scenedesmus.