Journal of Disease and Global Health
 

Journal of Disease and Global Health, ISSN No. : 2454-1842, Vol.: 10, Issue.: 1

Original Research Article

ALLERGIC IMPLICATIONS OF BLA G 1 AND BLA G 2 CONCENTRATIONS IN HOUSES OF SAUDI ARABIA

 

SYED M. HASNAIN1*, ALANOUD AL-QASSIM1, ABDULRAHMAN AL-FRAYH2, HUSSEIN AL-MEHDAR3, SULTAN AL-SEDAIRY1 AND FUTWAN AL-MOHANNA1

1Allergy and Medical Aerobiology Section, Department of Cell Biology, King Faisal Specialist Hospital and Research Centre, P.O. Box 3354, Riyadh 11211, Saudi Arabia.

2Department of Pediatric, College of Medicine, King Saud University, P.O. Box 7805, Riyadh 11472, Saudi Arabia.

3Department of Biology, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah 21589, Saudi Arabia.

Abstracts

Aims: As the higher cockroaches (CKRs) concentrations in houses are associated with allergic diseases, our objective was to determine the concentration levels of CKRs allergen.

Study Design: Quantitative and qualitative determinations of CKRs’ antigenic component in indoor environment of diagnosed allergic patients and non-allergic individuals.

Methodology: A total of 560 House Dust Samples (HDS) were randomly collected from patient and control houses and grouped under coastal and non-coastal regions. HDS were extracted in Phosphate Buffered Saline (PBS) and analyzed by ELISA using antibodies obtained from Indoor Biotechnologies, USA.

Results: Bla g 1 and Bla g 2, being two main allergenic components in Blattella germanica, constituted 42.3% and 35.5% respectively. Bla g 1 with 28.7% and 23.6% of Bla g 2 were present in control houses compared to 13.6% of Bla g 1 and 11.4% of Bla g 2 in patient houses. A comparison of data resulted in 7.3% of Bla g 1 and 5.3% of Bla g 2 in coastal compared to 35.3% and 29.4% in non-coastal regions. As the clinical threshold levels of both allergens are different, Bla g 2 marginally higher (2.3 µg/g) in patient than control samples (1.8 µg/g). By contrast, Bla g 1 was less prevalent in patient (1.5 U/g) compared to control houses (16.5 U/g).

Conclusion: There appear to be no major variation in the concentration levels of Bla g 1 and Bla g 2 between patients and control houses. Nevertheless, Bla g 2 was found to be marginally higher in patients’ samples.

Keywords :

Cockroaches; allergic diseases; asthma.