Journal of International Research in Medical and Pharmaceutical Sciences
 

Journal of International Research in Medical and Pharmaceutical Sciences, ISSN No. : 2395-4477 (Print), 2395-4485 (Online), Vol.: 11, Issue.: 4

Original Research Article

ISOQUINOLINE ALKALOID BERBERINE ACTS AS AN INHIBITOR OF OSTEOCLAST DIFFERENTIATION

 

PETYA GANOVA1, VALERIYA GYURKOVSKA1, TSVETELINA DONCHEVA2 AND NINA IVANOVSKA1*

1Department of Immunology, Institute of Microbiology, Bulgarian Academy of Science, 1113 Sofia, Bulgaria.

2Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Science, 1113, Sofia, Bulgaria.

Abstracts

Objective: Berberine, a naturally occurring isoquinoline alkaloid, possesses a wide range of pharmacological activities, including anti-bacterial, anti-fungal, anti-protozoal, anti-inflammatory, anti-diabetes and anti-tumor properties. The purpose of this study was to investigate the effect of berberine on osteoclast differentiation of bone marrow (BM) cells obtained from BALB/c mice with zymosan-induced arthritis (ZIA).

Materials and Methods: Osteoclast precursors were stimulated for 6 days under different schemes and osteoclast formation was assessed by counting the number of tartrate-resistant acid phosphatase (TRAP)-stained multinuclear cells. The expression of receptor activator of nuclear factor κB ligand (RANKL), tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) and TRAIL-R2 (DR5) on cultivated cells were determined by flow cytometry.

Results: Berberine inhibited macrophage colony-stimulating factor (M-CSF)+RANKL-induced osteoclast differentiation and also, RANKL-independent osteoclast generation. Anti-osteoclastogenic effect of the alkaloid was related with markedly inhibited secretion of interleukin IL-1 and IL-6, with a decrease of RANKL expression on M-CSF+RANKL-stimulated BM cells, and with a lower expression of DR5.

Conclusion: Our data reveal the mechanisms of blocking osteoclastic differentiation by berberine and it thus holds promise to be further investigated in vivo as a therapeutic or preventive agent for bone-related diseases such as arthritis.

Keywords :

Berberine; osteoclasts; cytokines; interleukins.