Journal of Biochemistry International
 

Journal of Biochemistry International, ISSN No. : 2454-4760, Vol.: 4, Issue.: 1

Original Research Article

FREQUENCY OF ADIPONECTIN GENE POLYMORPHISM IN TYPE 2 DIABETES MELLITUS

 

HEND M. ABDELGHANY1, SALAMA R. ABDELRHEIM1, MOHAMMAD SAYYED BEKHEET2*, NILLY H. ABDALLA3 AND RASHA F. AHMED1

1Department of Biochemistry, Faculty of Medicine, Minia University, Egypt.

2Department of Biochemistry, Faculty of Medicine, Al Azhar University, Egypt.

3Department of Internal Medicine, Faculty of Medicine, Benisuef University, Egypt.

Abstracts

Reduced plasma levels of adiponectin have been demonstrated in individuals with obesity, insulin resistance and type 2 diabetes mellitus (T2DM) and may be used to predict the overall risk of developing insulin resistance and T2DM.

Recently, genome-wide scans in humans have mapped a susceptibility locus for T2DM and metabolic syndrome to chromosome 3q27, where the gene encoding adiponectin is present. A single nucleotide polymorphism 45(SNP45) T/G was found to be associated with obesity and insulin resistance.

The aim of the present study was to examine the correlation between the SNP45 T/G polymorphism in the adiponectin gene and the increased risk of T2DM in El Minia governorate, Egypt.

The genotype frequency of SNP45 T > G in exon 2 of adiponectin gene was determined by PCR based restriction enzyme analysis using the restriction enzyme called SmaI.  

The results of this study showed a significant increase in mutant TG/GG genotype in T2DM patients (36.7%) in comparison to control group (18%) (P value < 0.05). Subjects with the G/G genotype were at increased risk for T2DM compared with those having the T/T genotype. There is a significant increase in mutant G allele in T2DM patients group (22%) in comparison to control group (12%) (P value˂0.05). No significant relation between genotypes of adiponectin and clinical data of the patients (age, sex, BMI, FBS, and HbA1c).

In conclusion, the present study supports the association between adiponectin +45 T/G single nucleotide polymorphism (SNP) with increased risk of type 2diabetes mellitus.

Keywords :

Polymorphism; adiponectin; type 2 diabetes mellitus.