Journal of Applied Physical Science International
 

Journal of Applied Physical Science International, ISSN No. : 2395-5260 (Print), 2395-5279 (Online), Vol.: 9, Issue.: 1

Original Research Article

CHARACTERIZATION OF CONFORMATIONAL CHANGES OF EXTRACELLULAR POLYMERIC SUBSTANCES (EPS) IN BULK SOLUTION AT ACID AND BASE pH USING FLORESCENCE MEASUREMENTS TECHNIQUE

 

ABUBAKER K. ELAYATT1*, EL FATEH BELKASEM2, ABDELQADER IMRAGAA2 AND KHALED EDBEY2

1Department of Chemical Engineering, Faculty of Engineering, University of Sirte, Libya.

2Department of Chemistry, Faculty of Science, University of Benghazi, Libya.

Abstracts

The conformational changes of extracellular polymeric substances (EPS) extracted from Pseudomonas putida ATCC 11172 at different pH values in solution where studied using fluorescence spectroscopy. The EPS was labeled using 8-aminopyrene-1,3,6-trisulfonic acid, tri sodium salt (APTS). The interactions between APTS and EPS surface in dilute aqueous solution were monitored using fluorescence spectroscopy. Steady state, excited state and time-resolved anisotropy measurements (TRAMS) were used to provide information about the changes in conformational behavior of the APTS as they bind to EPS surfaces.

Keywords :

Extracellular polymeric substances; fluorescence steady state measurement; Steady State technique; time resolved anisotropy measurements.