PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY
 

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, ISSN No. : 0972-2025, Vol.: 18, Issue.: 7-8

Original Research Article

ASSESSMENT OF VIABILITY AND ENHANCEMENT OF GERMINATION POTENTIAL OF JOJOBA SEEDS UNDER In vitro CONDITIONS USING PLANT GROWTH REGULATORS

 

RAMAN BALA*, JITENDER SINGH LAURA AND VIJAY SINGH BENIWAL

Department of Environmental Sciences, Maharshi Dayanand University, Rohtak, Haryana-124001, India [RB, JSL].

Department of Horticulture, Chaudhary Charan Singh Haryana Agriculture University, Hisar, Haryana-125001, India [RB, VSB].

Abstracts

Jojoba [Simmondsia chinensis (Link) Schneider] produces a unique liquid-wax which has commercial potential. The present investigation was carried out to assess viability and in vitro germination tests of fresh and one-year-old seeds, stored in darkness at room temperature conditions. Viability of seeds was determined using Tetrazolium (TZ) test. It was found that light (16/8 h day/night photoperiod) conditions resulted in early and higher seed germination percentage as compared to total dark period under in vitro culture conditions. Improvement in germination percentage of one-year -old seeds was observed using Gibberellic acid (GA3), Benzylaminopurine (BAP) and Thidiazuron (TDZ) under given photoperiod conditions. Interestingly, TDZ 1.0 mgL-1 concentration produced highest germination rate of seeds (92%), as well as better seedling growth, followed by BAP with 70% germination rate at 0.5 mgL-1 concentration.

Keywords :

Gibberellic acid; light; liquid-wax; Simmondsia chinensis; tetrazolium test; thidiazuron.