PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY
 

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, ISSN No. : 0972-2025, Vol.: 19, Issue.: 3-4

Original Research Article

A NEW SIMPLE AND EFFICIENT DNA EXTRACTION PROTOCOL FOR ORCHID WITHOUT LIQUID NITROGEN AND PHENOL

 

JOYRISON KAMBA AND CHITTA RANJAN DEB*

Department of Botany, Nagaland University, Lumami 798627, Nagaland, India [JK, CRD].

Abstracts

With the advancement of molecular biology techniques many conventional genomic DNA extraction protocols has been developed for good, pure, high quality DNA with expensive and hazardous chemical reagents. Here we present a rapid, simple and efficient DNA extraction protocol using cetyl trimethylammonium bromide (CTAB) for orchid without involvement of liquid nitrogen and toxic phenols which are environmentally hazardous. The extracted genomic DNA was successfully used for Polymerase Chain Reaction (PCR) amplification of plant barcode genes (rbcL, matK and ITS) for orchid species.

Keywords :

DNA extraction; CTAB; orchid; liquid nitrogen; phenol; PCR; barcode.