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High Frequency Plant Regeneration in Ashwagandha [Withania somnifera (L.) Dunal].: An Important Medicinal Plant

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 1-2 2003, Pages: 49-56

A high frequency and rapid regeneration protocol via callus and directly from various explants was developed in Withania somnifera (L.) Dunal. Callus was initiated from internodal segment, leaf, root and petiole explants on MS and B5 media supplement...

In Vitro Morphogenesis in cultivated varieties of Sorghum bicolor (L.) Moench

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 1-2 2003, Pages: 43-48

In vitro plant regeneration is achieved in cultivated varieties of Sorghum bicolor from immature inflorescence explants through embryogenic callus induction and also organogenesis. Explants were cultured on modified Murashige and Skoog's medium, supp...

Cloning and Expression analysis of Water Stress-Induced cDNAs from radicle of Indica Rice (Oryza sativa Cv. Nootripathu) by mRNA Differencial Display ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 1-2 2003, Pages: 33-42

The landraces of Indica rice (Oryza sativa L.) are well adapted to drought conditions throughout their life. Their superior germination and seedling root characters enable them to withstand drought during their early stages. To identify water stress-...

Epigenesis and High Frequency Plant Regeneration from Hypocotyl Explants of Soybean (Glycine max L. Merrill)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 1-2 2003, Pages: 23-32

High frequency somatic embryo induction was achieved in Glycine hypocotyls depending upon genotype and the explant inoculation medium. Plant regeneration efficiency was in excess of 85% following plural shoot initiation in genotype JS 90-41 when the ...

In Vitro Plant Regeneration from Nodal and Leaf Explants of Solanum surattense Burm. F. A Medicinal Plant

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 1-2 2003, Pages: 17-22

An efficient method was developed for in vitro propagation Solanum surattense Burm. f. Multiple shoot formation was noticed on Murashige and Skoog�s medium supplemented with 2 mg/l 6-benzylaminopurine and 1 mg/l kinetin from nodal explants and 4...

Enhanced Somatic Embryogenesis and Plant Regeneration in Bamboo (Dendrocalamus strictus)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 1-2 2003, Pages: 9-18

Enhanced somatic embryogenic system was developed using zygotic embryos of Dendrocalamus strictus. Linsmeir and Skoog (LS) medium supplemented with 27.14?M 2,4-D was suitable for the induction of somatic embryogenic callus. Proliferation of embryogen...

Biotechnology of Sandalwood - Current Trends and Future Needs

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 1-2 2003, Pages: 1-8

Sandalwood is a commercially important aromatic tree, and is the source of fragrant heartwood and oil. Conventionally, new trees are raised through seeds and vegetative propagation has not been successful in this species. Multiplication of geneticall...

In Vitro Production of Rice Plants from Synthetic Seeds by Encapsulation of Somatic Embryos

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 155-158

Somatic embryos of rice [Oryza sativa (cv.Tellahamsa)] were encapsulated in an alginate matrix to produce synthetic seeds. Eight to ten-day-old globular somatic embryoids were found suitable for germination and plant conversion. In the present invest...

Callus Formation and Shoot Regeneration from Mature and Juvenile Explants of Murraya koenigii (Spreng)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 151-154

Murraya koenigii is a tree cultivated for its fragrant leaves used for culinary and medicinal purposes. Callus could be induced only from the internodes of mature plants. Morphogenetic callus was induced with BAP alone or in combination with NAA. The...

In Vitro Production of Virus Free Plants in Citrus reticulate

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 147-150

Virus free plantlets were successfully produced using meristem tip as explants. Meristem dome with 2 leaf primordial (Size 100-200u) was cultured in modified MS liquid media (M19) containing 4 mg l-1 Thamine, 0.01mg l-1 Riboflavin and 0.01 mg. l-1 GA...

Regeneration in Chenopodium album L.via Organogenesis and Somatic Embryogenesis

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 143-146

Mode of plant regeneration from callus was studied in Chenopodium album L. (2n=18; broad leafed cytotype), a semi cultivated crop rich in ?-carotene. Scanning electron microscopic study revealed both organogenesis and somatic embryogenesis from the s...

In Vitro Callus Induction and Plantlet Regeneration from Indian Cotton Cultivars

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 137-142

Efficient plant regeneration from callus cultures of Indian cotton cultivars is reported here. Ten Indian cotton cultivars namely NCS3, NA920, NHH390, NA1325, PMC, LRA, MCU5, DCH32, Srisailam and Anjali were used for the present experiment. Different...

In Vitro Plant Regeneration from Leaf Sheath Cultures of Sugarcane via Organogenesis

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 131-136

In vitro morphogenesis was obtained from leaf sheath derived calli of three local varieties ( Isd-16, Isd-28 and Amrita ) of sugarcane (Saccharum officinarum L). The calli induced in 2,4-D (0.5mg./l) containing MS medium showed better regenerating ca...

Agrobacterium-mediated Genetic Transformation of Chickpea (Cicer arietinum L.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 125-130

Transformation of chickpea (Cicer arietinum L.) was achieved using Agrobacterium tumefaciens strain EHA105 harboring binary vector p35SGUSINT containing uidA (GUS) and npt II genes, and pJB90GI containing uid A (GUS) and hpt genes. A strong GUS expre...

Microsatellite Markers for Yellow Stem Borer (Scirpophaga incertulas Walker.) Resistance in Rice

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 117-124

The availability of molecular markers for various traits is a prerequisite for Marker Assisted Selection, Map based cloning etc. The possibility to tag genes for yellow stem borer resistance with microsatellite markers was investigated in the present...

In Vitro Plant Regeneration from Shoot Tip Explants of Panicum sumatrence

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 111-116

In vitro raised shoot tip explants from little millet were used to produce multiple shoots on a medium containing various concentrations of growth regulators. Among the combinations used, MS medium containing BAP (0.3 mg/l) and KIN (0.2 mg/l) combina...

Biotechnology of Sesame-An Oil Seed Crop

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 101-110

Sesamum (Sesamum indicum) is an ancient oil seed crop and grown in India from antiquity. The primary aim of sesame breeding is to improve yield as well as oil content. But, both biotic and abiotic stress factors are hampering the realization of the f...

Somatic Cell Genetics in Improvement of Tree Species

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 81-100

Nucellar Embryogenesis and Artificial Seed Production in Citrus reticulata.

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 1-2 2002, Pages: 77-80

A study was undertaken to develop a protocol for nucellar embryogenesis and artificial seed preparation in Khasimandarin. Nucellar embryoids were formed in 35 days after culturing in NE4 media containing MT basal with malt extract (500mg l-1) (W/V)BA...

Micropropagation of Tylophora asthmatica and Uraria picta

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 1-2 2002, Pages: 73-76

Tylophara asthmatica and Uraria picta are medicinal plants finding a wide range of applications in the Ayurvedic system of medicine. Nodal explants of these two plants were cultured on MS medium supplemented with BAP for shoot bud induction. The maxi...