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Shoot Bud Induction and Somatic Embryogenesis in Callus Cultures of Munudlea sericea (Wild.) A. Cheval.

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 149-154

Cotyledonary segments cultured on MS medium fortified with various combinations of auxins like Dicamba, IAA, IBA and NAA produced compact brown callus, which later on developed into greenish white shoot buds. These shoot buds on transferring to the s...

Micropropagation of Nendran and Poovan varieties of Musa through Inflorescence Tip Culture

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 141-148

Determinate male inflorescence tips of nendran cultivar variety of banana cultured on modified MS medium supplemented with 4% sugar and 3 mg/l BAP induced normal buds within 120 days of culture. Indeterminate male inflorescence tips of poovan cultiva...

In Vitro Embryo Culture studies in Papaya

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 135-140

Papaya (Carica papaya L.) being a delicious fruit has the problem of sex expression. On segregation, the gynodioecious cultivar segregates in the ratio of 1 (female); 2 (heterozygous andromonoecious): 1 (homozygous dominant andromonoecious) forms. Th...

Effect of Heavy Metals on Somatic Embryogenesis in Solanum melongena L.

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 129-134

Somatic embryogenesis by the addition of heavy metals like Zn and Cu individually and in combination with cadmium was studied at different levels of these metals in MS media supplemented with 10 mg/l NAA. Absence of these two micro essential elements...

Plant Regeneration from Immature Cotyledon derived Cultures of Soybean (Glycine max L. Merr)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 121-128

High frequency morphogenic calli induction was attained in Glycine max from immature cotyledons. Eleven genotypes and three media were tested for in vitro morphogenic efficiency. The morphogenesis was dependent upon genotype and the explants inoculat...

High Frequency Plant Regeneration from Leaf Callus Cultures of Embelia ribes Burm. - A threatened Medicinal Plant

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 115-120

An efficient protocol for rapid regeneration of plantlets have been standardized through leaf callus culture of Embelia ribes Burm.- a threatened woody medicinal shrub of the Western Ghats. Tender leaf segments proliferate into luxuriant mass of call...

In Vitro Response of Immature Zygotic Embryos of Trifolium alexandrinum (Egyptian clover)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 109-114

Egyptian clover (Trifolium alexandrinum) has limited genetic variability. Efforts to induce genetic variability through interspecific hybridization in the species have failed due to incompatibility barriers. Present study was taken up to identify the...

Biochemical and Molecular Studies during Ontogeny of Somatic Embryos in Sandal (Santalum album L.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 101-108

Biochemical and molecular analyses were carried out at the sequential stages of somatic embryogenesis in Santalum album to understand whether the developmental anomalies have any genetic basis. The internodes collected from sandal trees of Marayoor p...

Multiple Shoot Induction in Capsicum annuum L. Cv. Early California Wonder

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 95-100

A rapid and reproducible regeneration protocol has been established in Bell pepper (Capsicum annuum L. cv. Early California wonder) via in vitro shoot multiplication. Multiple shoots were induced from shoot tip explants cultured on MS medium fortifi ...

Multiple Shoot Induction and Plant Regeneration of Peanut (Arachis hypogaea L.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 89-94

This study was designed to standardize the effect of picloram on plant regeneration from cotyledon explants of groundnut. High frequency of callus formation was noticed when the explants was cultured on MS medium with picloram. Three-week-old calli w...

In Vitro Plant Regeneration from Shoot Meristem Explants of Wheat (Triticum aestivum L.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 85-88

A simple procedure for high frequency multiple shoot regeneration from shoot meristem of wheat (Triticum aestivum L.) has been developed. Shoot apices from 1-week-old aseptically grown seedlings were cultured in vitro on MS medium supplemented with e...

Assessment of Genetic Diversity and Population Discrimination in Hypericum hookerianum of Western Ghats using RAPD

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 81-84

The genetic diversity in Hypericum hookerianu collectd from six different sites in Western Ghats was assessed. The aim was to compare inter and intra population genetic distances and to find out the areas of maximum genetic diversity. Out of 40 prime...

In Vitro Plant Regeneration from Stem Explants of Phaseolus vulgaris L

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 77-80

In vitro regeneration of shoot buds and roots from stem explants of the French bean, isolated from 5-6-day-old seedlings was studied under defined cultural conditions. Stem explants were cultured on MS medium supplemented with various concentrations ...

In Vitro Shoot Multiplication in Rosa indica L.

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 73-76

A rapid and reproducible regeneration protocol has been established in Rosa indica via nodal shoot multiplication. Nodes of 0.5 cm long cultured on MS medium supplemented with IAA and BA produced multiple shoots. After 30 days the shoots began yellow...

Interspecific Hybridization in genus Helianthus through Embryo Rescue

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 65-72

In a direct approach to rescue interspecific hybrids, immature embryos of cv. Morden were cultured at different developmental stages i.e. 1-4 weeks after fertilization on four percentage. Maximum germination percentage was observed from 4-weeks-old e...

Study of Androgenesis using Morphometrics and Regeneration in Indian Mustard (Brassica juncea Czern. and Coss.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 59-64

Anther culture involves identification, isolation and culture of appropriate stage of microspore, which world induce callus /embryo and subsequently give rise to androgenic plant. Anther culture of yellow seeded mustard (Brassica juncea) was carried ...

Effect of Fungicides and Antibiotics to control Microbial Contamination in Hevea Cultures

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 51-58

In vitro toxicity and microbial contamination control efficiency of three fungicides- benomyl, bavistin and fytolan and three antibiotics-nystatin, amphoterincin-B and streptomycin were assessed by incorporating them in the Hevea. shoot culture mediu...

Influence of the Physiological Age and Position of the Nodal Explants on Micropropagation of Field-Grown Dendrocalamus strictus Nees ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 45-50

An efficient and reproducible protocol for plant regeneration through in vitro culture of Dendrocalamus strictus is reported. Murashige and Skoog (MS) basal medium supplemented with N6-benzyladenine and adenine sulphate resulted in high frequency of ...

Effect of Age of Explants on Somatic Embryogenesis and Plant Regeneration in Eucalyptus tereticornis SM

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 39-44

Plant regeneration was achieved via somatic embryogenesis in Eucalyptus tereticornis SM. Callus was induced from cotyledon explants isolated from 7-to-28 day-old seedlings at weekly intervals when cultured on MS basal medium with different concentrat...

In Vitro Regeneration of Celastrus paniculatus Willd. -A Rare Medicinal Plant

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 33-38

In vitro regeneration protocol through the stem callus culture of Celastrus paniculatus Willd was established. The explants induced callus on LS medium supplemented with 2% fructose, 6 uM Kn and 1 uM IBA. Interaction of the same hormones at lower con...