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STUDIES ON IN VITRO MORPHOGENESIS FROM BULB SCALE AND LEAF DISC EXPLANT OF AMARYLLIS BELLADONNA L  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 14, Issue: 1-2 2013, Pages: 22-32

An efficient method for rapid propagation of Amaryllis belladonna L. has been developed in this study. Bulb scales and leaf discs were used as explants and cultured on MS medium amended with diverse auxins and cytokinins in varying concentrations as ...

PLANT REGENERATION FROM EXPLANTS OF DELONIXELATA L. VIA ORGANOGENESIS  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 14, Issue: 1-2 2013, Pages: 12-21

In this paper we report the establishment of Delonix elata regeneration through organogenesis. We used node old tree, seedlings shoot tip, leaf and stem explants excised from, Murashige and Skoog (MS) medium supplemented with various concentrations o...

MICROCLONING STUDIES IN ALOE BARBADENSIS  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 14, Issue: 1-2 2013, Pages: 1-11

Stem disc explant of Aloe was cultured on MS basal medium containing different auxins, cytokinins as sole as well as in combinations. Culture medium MS2D (MS+2.0 mg.l-1 2,4-D) initiated callus in higher proportion. Proliferation medium MS3B (MS+3.0 m...

Plant Regeneration and In Vitro Flowering from Nodal Explants of Hydrolea zeylanica - A Rare Medicinal Plant  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 3-4 2012, Pages: 147-150

An efficient micropropagation and in vitro flowering protocol was developed for an important and rare medicinal plant Hydrolea zeylanica. Multiple shoots regeneration from nodal explants was achieved on MS medium containing different concentration of...

Biochemical analysis between In Vivo and In Vitro Regenerated Plant Leaves of Solanum nigrum L.  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 3-4 2012, Pages: 141-146

To compare the efficacy for biochemical analysis of in vitro propagated plants and field grown plants of Solanum nigrum L. Maximum shoot multiplication were observed MS medium containing 0.75 mg/l BAP+0.5 mg/l IBA and 0.1 mg/l NAA. Immature tissues s...

Production of Aristolochic Acid (AA) I and II from Aristolochia indica and Aristolochia bracteolate through In Vitro Culture  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 3-4 2012, Pages: 131-140

Callus of Aristolochia indica and Aristolochia bracteolata was induced on MS basal medium supplemented with 3.0 mg/l and 1.0 mg/l of NAA from leaf explants of A. bracteolata and A. indica respectively. Aristolochic acid (AA) I and II were identified ...

In vitro Plant Regeneration via Direct Organogenesis from Shoot Tip Explants of Cucumber (Cucumis sativus L. Green long)  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 3-4 2012, Pages: 125-130

The present study is focused on induction of multiple shoots from shoot tip explants of cucumber. Shoot tip explants were excised from 7-day-old seedlings and cultured on MS medium fortified with different concentrations of (0.5-2.5 mg/l) BAP alone a...

Isolation, Characterization and Detection of Cry Gene Contents from South Indian Isolates of Bacillus thuringiensi  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 3-4 2012, Pages: 111-124

Bacillus thuringiensis Berliner based bio pesticides have been successfully used worldwide for the control of agricultural pests and vectors of human diseases; constitutes a large family of bacterial subspecies highly specialized as insect pathogens ...

In Vitro Plant Regeneration from Catharanthus roseus L. and Antibacterial activity of Leaves against Human Pathogens  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 3-4 2012, Pages: 105-110

Catharanthus roseus L. (periwinkle) is an important medicinal plant for novel pharmaceuticals since most of the bacterial pathogens are developing resistance against many of the currently available anti microbial drugs. To identify the antibacterial ...

In Vitro Clonal Propagation in Saraca asoca (roxb.) De Wilde: A Vulnerable Medicinal Plant  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 3-4 2012, Pages: 99-104

Near-isogenic wheat lines carrying various rust resistance genes were analysed for changes in various biochemical parameters. Rust Saraca asoca (Roxb.) de Wilde (Ceasalpiniaceae) is a vulnerable medicinal as well as ornamental potential tree, distrib...

Evaluation of the Genetic Fidelity of In Vitro Propagated Natural Sweetener Plant (Stevia rebaudiana Bert.) Using DNA-Based Markers  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 3-4 2012, Pages: 93-98

The genetic fidelity of in vitro-raised Stevia rebaudiana clones was assessed by using Random Amplified Polymorphic DNA (RAPD) analysis. A total of 40 RAPD primers were used for initial screening with the mother plant of Stevia but only 29 RAPD decam...

Plant Mediated Synthesis of Silver Nanoparticles by using Leaf Extracts of Dioscorea oppositifolia L. and their Antimicrobial Activity  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 3-4 2012, Pages: 83-92

Plant mediated synthesis of metallic nanoparticles is an increasing commercial demand due to the wide applicability in various areas such as electronics, catalysis, chemistry, energy, cosmetics and medicine. In the present investigation, synthesis of...

Molecular Markers in Bamboo Systematics and Germplasm Screening  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 3-4 2012, Pages: 73-82

Bamboos are tall, arborescent, grasses which are put to over 1500 documented uses. Besides serving the rural community through multiple uses, they are extensively used as raw material for paper industry. The immense utility and versatility of this na...

A Rapid Protocol for In Vitro Multiplication from Shoot Tip Explants of Tanacetum cinerariaefolium: An Important Insecticide Producing Medicinal Plant  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 1-2 2012, Pages: 69-72

Pyrethrum, Tanacetum cinerariaefolium (Trevir.) Schultz-Bip, Asteraceae is a source of the natural, non-persistant, an ecofriendly insecticide pyrethrine. As their overexploitation in phytopharmaceutical industry is increasing, there is a need for de...

In Vitro Micropropagation of Tylophora indica (Burm.F) Merril Through Shoot Tip Explants  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 1-2 2012, Pages: 65-68

Clonal propagation of Tylophora indica from shoot tip explants placed on MS medium with low concentration of BAP (1 �M) and in combination with Ads (3 �M) induced three shoots and four roots. Explants on MS with BAP (4 �M) and KIN (3 ...

Efficient Method for In Vitro Plant Regeneration in Bambusa nutans Wall. Ex Munro Using Nodal Explants from Field-Grown Culms  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 1-2 2012, Pages: 59-64

A rapid and reproducible in vitro regeneration protocol for Bambusa nutans a multipurpose bamboo species has been developed using nodal explants collected from the lateral branches of field grown tree. Nodal explants were cultured on Murashige and Sk...

Agrobacterium-mediated Transformation and Plant Regeneration from Different Explants of Lycopersicon esculentum  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 1-2 2012, Pages: 53-58

Tomato is one of the most important and essential vegetable crops all over the world. The present study was focused on genetic transformation and plant regeneration of tomato (Lycopersicon esculentum cv PKM-1). Transformation was carried out by Agrob...

RAPD Markers for Identification of Stable CMS and Restorer Lines of Pigeonpea (Cajanus cajan)  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 1-2 2012, Pages: 47-52

To achieve success in hybrid seed production, it was essential to develop cytoplasmic genetic male sterility system (CMS) that would be acceptable and feasible to both farmers as well as commercial hybrid seed producer. Identification of pigeonpea CM...

Development of an Efficient Protocol for High Frequency Plant Regeneration from Nodal Explants of Solanum nigrum L.  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 1-2 2012, Pages: 41-46

An efficient protocol was devised for rapid propagation of Solanum nigrum L. from nodal explants. The nodal explants were cultured on MS medium supplemented with different concentrations (0.5-2.5 mg/l) of BAP and KIN for Multiple shoot bud induction....

Effect of NaCl Treatment on In Vitro Shoot Multiplication of Brassica juncea (L.)  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 1-2 2012, Pages: 35-40

The present experiment was focused to study the effect of different concentrations of NaCl on shoot multiplication in Brassica juncea. In vitro culture was established on MS medium supplemented with 1.0 mg/l BAP. NaCl at various concentrations was ad...