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Somatic Embryogenesis and Plant Regeneration of a Rare and Multipurpose Medicinal Plant Hemidesmus indicus (L.) Schult  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 71-74

Hemidesmus indicus (L.) Schult. (Family: Periplocaceae) is an important, multipurpose and a rare medicinal plant. It is locally known as Anantmul and is quite familiar as Indian Sarsaparilla. Protocol for in vitro plant regeneration from callus via s...

Plant Regeneration through Leaf Culture of Lycopersicon esculentum Mill  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 67-70

Tomato (Lycopesicon esculentum cv. Pusa Ruby) leaf explants were cultured on MS medium supplemented with different concentrations of BAP/Kn along with 0.5/1.0 mg/l IAA. High percentage of response was observed at 1.0 mg/l IAA + 5.0 mg/L BAP. More num...

In vitro Propagation of Erianthus arundinaceus by Shoot Tip Culture  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 61-66

A protocol was developed for in vitro propagation of Erianthus arundinaceus clones. Shoot tips of Erianthus after sterilization were subjected to vertical split before inoculation. The best response for shoot proliferation was attained in the MS medi...

Effect of Genotypes and Plant Growth Regulators on in vitro Morphogenesis in Muskmelon (Cucumis melon L.) From Cultured Mature Cotyledons  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 45-60

Mature cotyledons of four cultivars of Cucumis melon were cultured on twenty different fortifications of MS media to assess their in vitro response. Higher in vitro morphogenesis leading to plantlet regeneration was varied considerably due to genotyp...

Isolation of Cell Lines Resistant to Erysiphae cichoracearum by Screening Different Genotypes of Cucumis sativus L.  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 39-44

Leaf derived calli of four genotypes of cucumber susceptible, moderately resistant and resistant plants were screened in vitro for the production of powdery mildew disease (Erysiphae cichoracearum). Three methods were used to get resistance i.e., dir...

High Root Biomass Production in Auxin- Supplemented Cultures of Withania somnifera (Dunal)  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 31-38

Plants remain as indispensable source for a number of chemicals substances that are difficult to synthesize chemically owing to complicated structures. For the various reasons, the cultured cell and organs of higher plants are preferred over the conv...

Asymbiotic Seed Germination and Seedling Development in an Endangered and Commercially Important Orchid-Vanda coerulea Griff Ex. Lindl.  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 25-30

The immature embryos of Vanda coerulea, obtained from undehisced green capsules (28 wap) were cultured on M medium and its supplementation with plant growth regulators (NAA, IBA, BAP; each used at 1.0 mg/l). Sixty percent seeds germinated within 4 we...

In vitro Regeneration from Epicotyl explant of Caesalpinia decapetala (Roth.)  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 19-24

An efficient protocol was developed for in vitro shoot regeneration from an aseptically grown seedling of Caesalpinia decapetala (Roth.) Alston using epicotyls explant on MS medium supplemented with BA (1.0 mg/l). It induced maximum number of multipl...

Plant Regeneration from Nodal Segments in Phyllanthus amarus Schum and - An Important Medicinal Plant  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 13-18

A plant regeneration protocol developed for Phyllanthus amarus Schum and Thonn; it is an important major raw material for Chavanprash (a worldwide used ayurvedic preparation) and a source of hepatoprotective chemicals phyllanthin and hypophyllanthin....

Direct Somatic Embryogenesis in Gloriosa superba L. An Endangered Medicinal Plant of India  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 7-12

Gloriosa superba L., is a valuable endangered medicinal plant where the corms are used for in vivo multiplication. One corm gives only one or at the maximum two plants a year. The present study reports that somatic embryogenesis directly from leaf ex...

Effect of Different Carbohydrates on Plant Regeneration in Bacopa monniera (L.) Pennel  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 1-6

The effects of different carbon sources such as glucose, galactose, mannose, sucrose, lactose, starch, mannitol and sorbitol were studied on plant regeneration in Bacopa monniera L. Pennel. Shoot multiplication and rhizogenesis was significantly high...

Genetic Transformation in Brassica juncea L. using Wild Types and Shooter Mutants of Agrobacterium tumefaciens and the Regeneration Response  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 3-4 2007, Pages: 205-208

Different wild types of Agrobacterium tumefaciens and their shooter mutants were used for infection of in vitro grown Brassica juncea and the resultant tumour / tumour infection sites were cultured on Murashige and Skoog s basal medium supplemented w...

In Vitro Plant Regeneration and Flowering of Physalis minima L. :A Medicinal Plant  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 3-4 2007, Pages: 201-204

A protocol for in vitro regeneration and flowering was established in Physalis minima L. High frequency and maximum number of multiple shoots were obtained from leaf as well as stem explants on M.S medium supplemented with 3.0 mg/l BAP & 0.5 mg/l...

RAPD based Genetic Diversity analysis in Banana Hybrids  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 3-4 2007, Pages: 197-200

A total of 10 random primers were evaluated for banana hybrids based on RAPD polymorphism. Diversity analysis carried out by using RAPD markers in phase I hybrids revealed the confirmation of the hybridization along with the phylogenetic relationship...

RAPD based Genetic Diversity analysis in Banana Hybrids  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 3-4 2007, Pages: 193-196

A total of 10 random primers were evaluated for banana hybrids based on RAPD polymorphism. Diversity analysis carried out by using RAPD markers in phase I hybrids revealed the confirmation of the hybridization along with the phylogenetic relationship...

Micropropagation of Vetiver Grass (Vetiveria zizanoides L. Nash): A Potential Plant for Environmental Conservation  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 3-4 2007, Pages: 193-196

High frequency shoot multiplication was obtained from isolated shoot tips of vetiver grass (Vetiveria zizanoides L. Nash) cultured on Murashige and Skoog's basal medium supplemented with benzyladenine and a-naphthalene acetic acid. When different con...

PCR methods for the Distinction of some Wild and Cultivated species of Bauhinia  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 3-4 2007, Pages: 179-186

The genus Bauhinia L., is very variable in its habit and includes trees, lianas, and shrubs. These plants are very popular in warm regions for their gorgeous, orchid-like blossoms. The flowers exhibit enormous color variations both within and between...

Rapid Micropropagation of Cassia siamea  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 3-4 2007, Pages: 173-178

The present investigation deals with rapid micropropagation of Cassia siamea Lam. belonging to the family Caesalpiniaceae. This protocol was developed for multiplication by using shoot tip, cotyledonary node, and nodal explants derived from seedlings...

Conservation of Ceropegia oculata Hook. (Asclepiadaceae) by Micropropagation using Nodal Explants  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 3-4 2007, Pages: 167-172

An efficient in vitro shoot propagation method for starchy edible tuber producing asclepiad Ceropegia oculata Hook., categorized as an endangered and threatened in the Red Data Book of India and IUCN, was developed in this study. The cultures were es...

Influence of in vitro Mutagenesis on Ovule Culture and Plant Regeneration in Cotton (Gossypium hirsutum L.)  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 3-4 2007, Pages: 159-166

The effects of gamma rays, ethyl methanesulphonate (EMS) and sodium azide (SA) on ovule culture, growth rate of ovular callus and percentage of seedling regeneration were investigated. Based on preliminary experiments, 15 day-post-anthesis (dpa) ovul...