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A Study on Endogenous Level of Kanamycin Resistance of Sesame (Sesamum indicum L.)  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 3-4 2008, Pages: 135-138

Investigation was undertaken to study the endogenous level of Kanamycin resistance of Sesamum indicum genotypes under varied concentrations to Kanamycin antibiotic (0 to 150 mg/l). S. indicum genotypes were highly sensitive to Kanamycin exposure; see...

Role of Growth Regulators in Callus Establishment and Differentiation in Pluchea lanceolata (Dc.) C.B. Clarke  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 3-4 2008, Pages: 127-134

A protocol for callus establishment and differentiation in medicinally potent plant, Pluchea lanceolata has been developed by regulating the auxin-cytokinin ratio in basal media, source of explants and physiological state of the tissue in culture. Am...

Morphogenesis and Plantlet Regeneration from Leaf Disc Explants of Liquorice (Glycyrrhiza glabra L.)  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 3-4 2008, Pages: 117-126

A reliable and reproducible protocol has been developed for plant regeneration from 6-8 mm long leaf discs excised from 4 -5 months-old plants of Local cultivar Glycyrrhiza glabra L. The effect of optimum concentrations of different plant growth regu...

Evaluation of Salt Tolerance in the Salt-Tolerant Cell Lines and Regenerants of Vitis vinifera L. Cv. Tas-A-Ganesh  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 3-4 2008, Pages: 109-116

Callus cultures were established from axillary bud explants of grape (Vitis vinifera L.) cv. Tas-A-Ganesh on MS medium supplemented with 2.2 ?M 6-benzylaminopurine and 10.72 ?M ?-Napthalene acetic acid. NaCl-tolerant callus lines were obtained by exp...

Genetic Relationship Analysis in Rubber Tree (Hevea Brasiliensis Muell. Arg.) By DNA Fingerprinting  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 3-4 2008, Pages: 101-108

Development of molecular markers for various desirable traits like disease resistance, drought tolerance, compact canopy, higher yield, etc hold great potential for crop improvement in a perennial crop like rubber tree where conventional breeding pro...

In Vitro Embryo Culture and Ex Situ Regeneration Studies in Ancistrocladus heyneanus Wall. Ex Grah  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 3-4 2008, Pages: 95-100

Ancistrocladus heyneanus Wall. Ex Grah. (Ancistrocladaceae), an endemic Indian liana producing napthylisoquinoline alkaloids, was successfully multiplied in vitro through embryo culture. Attempt was also made to study habit, habitat, regeneration and...

Suspension Culture Studies in Lavandula bipinnata to check Secondary Metabolite Accumulation  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 3-4 2008, Pages: 89-94

Cultivation of plants is usually limited to specific areas and seasons, while due to plant tissue and cell cultures it is available wherever and whenever required. Thus desired products will be available constantly regardless of climate and place. Co...

Discrimination of Blue and White Flowered Clitoria ternatea Linn. (Fabaceae) Through RAPD assay  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 3-4 2008, Pages: 81-88

RAPD finger printing was used to measure the relatedness between the populations. RAPD primers were screened on a pool of genomic DNA of 16 samples of Clitoria ternatea bearing blue and white flowers. For each (both blue and white) variety, the DNA e...

In vitro Propagation of Ocimum americanum-A Medicinal Herb  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 3-4 2008, Pages: 75-80

The present work aims to establish a protocol for in vitro propagation of Ocimum americanum using inflorescence, leaf, nodal and internodal explants. Embryogenic callus was induced from all explants on MS medium fortified with 1 to 3.5 mg/l of 2,4-D....

Somatic Embryogenesis and Plant Regeneration of a Rare and Multipurpose Medicinal Plant Hemidesmus indicus (L.) Schult  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 71-74

Hemidesmus indicus (L.) Schult. (Family: Periplocaceae) is an important, multipurpose and a rare medicinal plant. It is locally known as Anantmul and is quite familiar as Indian Sarsaparilla. Protocol for in vitro plant regeneration from callus via s...

Plant Regeneration through Leaf Culture of Lycopersicon esculentum Mill  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 67-70

Tomato (Lycopesicon esculentum cv. Pusa Ruby) leaf explants were cultured on MS medium supplemented with different concentrations of BAP/Kn along with 0.5/1.0 mg/l IAA. High percentage of response was observed at 1.0 mg/l IAA + 5.0 mg/L BAP. More num...

In vitro Propagation of Erianthus arundinaceus by Shoot Tip Culture  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 61-66

A protocol was developed for in vitro propagation of Erianthus arundinaceus clones. Shoot tips of Erianthus after sterilization were subjected to vertical split before inoculation. The best response for shoot proliferation was attained in the MS medi...

Effect of Genotypes and Plant Growth Regulators on in vitro Morphogenesis in Muskmelon (Cucumis melon L.) From Cultured Mature Cotyledons  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 45-60

Mature cotyledons of four cultivars of Cucumis melon were cultured on twenty different fortifications of MS media to assess their in vitro response. Higher in vitro morphogenesis leading to plantlet regeneration was varied considerably due to genotyp...

Isolation of Cell Lines Resistant to Erysiphae cichoracearum by Screening Different Genotypes of Cucumis sativus L.  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 39-44

Leaf derived calli of four genotypes of cucumber susceptible, moderately resistant and resistant plants were screened in vitro for the production of powdery mildew disease (Erysiphae cichoracearum). Three methods were used to get resistance i.e., dir...

High Root Biomass Production in Auxin- Supplemented Cultures of Withania somnifera (Dunal)  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 31-38

Plants remain as indispensable source for a number of chemicals substances that are difficult to synthesize chemically owing to complicated structures. For the various reasons, the cultured cell and organs of higher plants are preferred over the conv...

Asymbiotic Seed Germination and Seedling Development in an Endangered and Commercially Important Orchid-Vanda coerulea Griff Ex. Lindl.  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 25-30

The immature embryos of Vanda coerulea, obtained from undehisced green capsules (28 wap) were cultured on M medium and its supplementation with plant growth regulators (NAA, IBA, BAP; each used at 1.0 mg/l). Sixty percent seeds germinated within 4 we...

In vitro Regeneration from Epicotyl explant of Caesalpinia decapetala (Roth.)  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 19-24

An efficient protocol was developed for in vitro shoot regeneration from an aseptically grown seedling of Caesalpinia decapetala (Roth.) Alston using epicotyls explant on MS medium supplemented with BA (1.0 mg/l). It induced maximum number of multipl...

Plant Regeneration from Nodal Segments in Phyllanthus amarus Schum and - An Important Medicinal Plant  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 13-18

A plant regeneration protocol developed for Phyllanthus amarus Schum and Thonn; it is an important major raw material for Chavanprash (a worldwide used ayurvedic preparation) and a source of hepatoprotective chemicals phyllanthin and hypophyllanthin....

Direct Somatic Embryogenesis in Gloriosa superba L. An Endangered Medicinal Plant of India  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 7-12

Gloriosa superba L., is a valuable endangered medicinal plant where the corms are used for in vivo multiplication. One corm gives only one or at the maximum two plants a year. The present study reports that somatic embryogenesis directly from leaf ex...

Effect of Different Carbohydrates on Plant Regeneration in Bacopa monniera (L.) Pennel  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 9, Issue: 1-2 2008, Pages: 1-6

The effects of different carbon sources such as glucose, galactose, mannose, sucrose, lactose, starch, mannitol and sorbitol were studied on plant regeneration in Bacopa monniera L. Pennel. Shoot multiplication and rhizogenesis was significantly high...