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In Vitro Regeneration of Centella asiatica L.

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 53-56

Tissue culture technique has been established as a useful ex-situ conservation strategy for endangered species. This paper describes in vitro regeneration of Centella asiatica L. (Urb.) (Apiacae) a threatened species and preliminary estimation of sec...

In Vitro Somatic Embryogenesis from Seedling Explants of Bean (Phaseolus vulgaris L.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 47-52

A rapid highly repetitive and efficient protocol was developed for inducing indirect somatic embryogenesis using seedling explants of bean (Phaseolus vulgaris L.) viz., immature cotyledon, mature cotyledon, stem and leaf segments. Explants were cultu...

An Efficient Protocol for Organogenic Callus Induction and Plant Regeneration in Tomato (Lycopersion esculentum Mill.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 41-46

Organogenic callus induction and plant regeneration was achieved by using hypocotyls, leaf and cotyledonary segments. From the obtained different forms of callus, the green compact and nodular callus only produced the multiple shoots. More amount of ...

In Vitro Plant Regeneration from Young Capitulum Explants of Gerbera jamesonii

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 35-40

An efficient protocol was developed for large-scale propagation using young capitulum explants in Gerbera jamesonii. Murashige and Skoog medium supplemented with 6- Benzylaminopurine at 7 mg/l in combination with 0.1mg/l indole-3-acetic acid was foun...

Effect of Various Factors on Plantlet Regeration of Curculigo orchioides Gaertn.

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 29-34

Curculigo orchiodes Gaertn. is an endangered anticarcinogenic and aphrodisiac herb. It is available during approximately four months of the monsoon season each year. Due to over exploitation of the plant for various medicinal uses, there is a tremend...

Agrobacterium-mediated Genetic Transformation and Regeneration of Transgenic Plants in Eucalyptus tereticornis

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 23-28

Transgenic plants were regenerated from transformed embryogenesis tissue of Eucalyptus tereticornis Sm. Friable nodular callus initiated from mature zygotic embryos on MS agar medium supplemented with 2mg/l NAA were transferred to MS medium containin...

Plant Regeneration and In Vitro Flowering from Leaf and Nodal Explants of Solanum nigrum (L.) - An Important Medicinal Plant ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 17-22

Direct Organogenesis and in vitro flowering was obtained in Solanum nigrum (L.). High frequency and maximum number of multiple shoots were obtained from leaf and nodal explants on MS-medium supplemented with BAP and IAA (2.0mg/l+1.0mg/l). Regenerates...

High Frequency Shoot Regeneration and Agrobacterium � Mediated DNA transfer in Red Chilli (Capsicum annuum L.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 9-16

Different explants of three cultivars of Capsicum annuum (K1,K2 and PLR 1) were tested for their regeneration response. Shoot tip explants were co-cultivated with Agrobacterium strain EHA 105 carrying a T-DNA with CaMV 35S promoter- gus marker gene f...

AFLP Fingerprinting of some Elite Indian Cotton Genotypes

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 1-8

Amplified Fragment Length Polymorphism (AFLP) analysis was used to evaluate the genetic diversity of some elite Indian cotton genotypes. Twenty cotton genotypes belonging to Gossypium hirsutum L., and three to G. arboreum L. were analyzed with nine A...

In Vitro Regeneration of Plants from Cotyledons of Crotalaria longipes Wight & Arn.- An Endangered Legume ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 173-176

Crotalaria longipes, an endangered plant threatened with extinction was regenerated from callus of cotyledonary explants of in vitro germinated seeds. Sulfuric acid-scarified seeds produced 96% germination in Murashige and Skoog (MS) (1962) basal med...

In Vitro Propagation of Jute Plants

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 169-172

Three different plants, viz. Jute (Corchorus olitorious; variety JRO-524), Dahlia sp. (winter annual), Heritiera fomes (halophyte), from three different eco-habital conditions were studied to understand the underlying mechanism of three fold checkpoi...

Agrobacterium -Mediated Genetic Transformation in Soybean (Glycine max (L.) Merr.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 165-168

An efficient and reproducible protocol for Agrobacterium-mediated transformation has been standardization in soybean (Glycine max (L.) Merr.) with a binary vector pBI 121. Putatively transgenic shoots were selected on kanamycin medium. GUS assay was ...

In Vitro Detection of Neem Oil (Azadirachta indica A.Juss.) in Seed derived Callus: An alternative Source of Future Promise ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 161-164

Callus initiated from germinating seeds of Azadirachta indica A. Juss was maintained on a modified Murashige and Skoog medium (MS). Organogenesis occurred in medium supplemented with various growth regulators. Undifferentiated callus tissues and seed...

Effect of Genotype and Culture Medium on Morphogenesis of Linseed (Linum usitatissimum L.) Immature Embryo Culture

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 155-160

Immature embryos of ten linseed genotypes were cultured on MS basal medium with 13 different combinations of growth hormones. Based on their in vitro response in preliminary experiment, two medium combinations were selected for the final experimentat...

Shoot Bud Induction and Somatic Embryogenesis in Callus Cultures of Munudlea sericea (Wild.) A. Cheval.

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 149-154

Cotyledonary segments cultured on MS medium fortified with various combinations of auxins like Dicamba, IAA, IBA and NAA produced compact brown callus, which later on developed into greenish white shoot buds. These shoot buds on transferring to the s...

Micropropagation of Nendran and Poovan varieties of Musa through Inflorescence Tip Culture

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 141-148

Determinate male inflorescence tips of nendran cultivar variety of banana cultured on modified MS medium supplemented with 4% sugar and 3 mg/l BAP induced normal buds within 120 days of culture. Indeterminate male inflorescence tips of poovan cultiva...

In Vitro Embryo Culture studies in Papaya

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 135-140

Papaya (Carica papaya L.) being a delicious fruit has the problem of sex expression. On segregation, the gynodioecious cultivar segregates in the ratio of 1 (female); 2 (heterozygous andromonoecious): 1 (homozygous dominant andromonoecious) forms. Th...

Effect of Heavy Metals on Somatic Embryogenesis in Solanum melongena L.

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 129-134

Somatic embryogenesis by the addition of heavy metals like Zn and Cu individually and in combination with cadmium was studied at different levels of these metals in MS media supplemented with 10 mg/l NAA. Absence of these two micro essential elements...

Plant Regeneration from Immature Cotyledon derived Cultures of Soybean (Glycine max L. Merr)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 121-128

High frequency morphogenic calli induction was attained in Glycine max from immature cotyledons. Eleven genotypes and three media were tested for in vitro morphogenic efficiency. The morphogenesis was dependent upon genotype and the explants inoculat...

High Frequency Plant Regeneration from Leaf Callus Cultures of Embelia ribes Burm. - A threatened Medicinal Plant

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 115-120

An efficient protocol for rapid regeneration of plantlets have been standardized through leaf callus culture of Embelia ribes Burm.- a threatened woody medicinal shrub of the Western Ghats. Tender leaf segments proliferate into luxuriant mass of call...