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Evaluation of the Genetic Fidelity of In Vitro Propagated Natural Sweetener Plant (Stevia rebaudiana Bert.) Using DNA-Based Markers  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 3-4 2012, Pages: 93-98

The genetic fidelity of in vitro-raised Stevia rebaudiana clones was assessed by using Random Amplified Polymorphic DNA (RAPD) analysis. A total of 40 RAPD primers were used for initial screening with the mother plant of Stevia but only 29 RAPD decam...

Plant Mediated Synthesis of Silver Nanoparticles by using Leaf Extracts of Dioscorea oppositifolia L. and their Antimicrobial Activity  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 3-4 2012, Pages: 83-92

Plant mediated synthesis of metallic nanoparticles is an increasing commercial demand due to the wide applicability in various areas such as electronics, catalysis, chemistry, energy, cosmetics and medicine. In the present investigation, synthesis of...

Molecular Markers in Bamboo Systematics and Germplasm Screening  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 3-4 2012, Pages: 73-82

Bamboos are tall, arborescent, grasses which are put to over 1500 documented uses. Besides serving the rural community through multiple uses, they are extensively used as raw material for paper industry. The immense utility and versatility of this na...

A Rapid Protocol for In Vitro Multiplication from Shoot Tip Explants of Tanacetum cinerariaefolium: An Important Insecticide Producing Medicinal Plant  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 1-2 2012, Pages: 69-72

Pyrethrum, Tanacetum cinerariaefolium (Trevir.) Schultz-Bip, Asteraceae is a source of the natural, non-persistant, an ecofriendly insecticide pyrethrine. As their overexploitation in phytopharmaceutical industry is increasing, there is a need for de...

In Vitro Micropropagation of Tylophora indica (Burm.F) Merril Through Shoot Tip Explants  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 1-2 2012, Pages: 65-68

Clonal propagation of Tylophora indica from shoot tip explants placed on MS medium with low concentration of BAP (1 �M) and in combination with Ads (3 �M) induced three shoots and four roots. Explants on MS with BAP (4 �M) and KIN (3 ...

Efficient Method for In Vitro Plant Regeneration in Bambusa nutans Wall. Ex Munro Using Nodal Explants from Field-Grown Culms  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 1-2 2012, Pages: 59-64

A rapid and reproducible in vitro regeneration protocol for Bambusa nutans a multipurpose bamboo species has been developed using nodal explants collected from the lateral branches of field grown tree. Nodal explants were cultured on Murashige and Sk...

Agrobacterium-mediated Transformation and Plant Regeneration from Different Explants of Lycopersicon esculentum  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 1-2 2012, Pages: 53-58

Tomato is one of the most important and essential vegetable crops all over the world. The present study was focused on genetic transformation and plant regeneration of tomato (Lycopersicon esculentum cv PKM-1). Transformation was carried out by Agrob...

RAPD Markers for Identification of Stable CMS and Restorer Lines of Pigeonpea (Cajanus cajan)  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 1-2 2012, Pages: 47-52

To achieve success in hybrid seed production, it was essential to develop cytoplasmic genetic male sterility system (CMS) that would be acceptable and feasible to both farmers as well as commercial hybrid seed producer. Identification of pigeonpea CM...

Development of an Efficient Protocol for High Frequency Plant Regeneration from Nodal Explants of Solanum nigrum L.  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 1-2 2012, Pages: 41-46

An efficient protocol was devised for rapid propagation of Solanum nigrum L. from nodal explants. The nodal explants were cultured on MS medium supplemented with different concentrations (0.5-2.5 mg/l) of BAP and KIN for Multiple shoot bud induction....

Effect of NaCl Treatment on In Vitro Shoot Multiplication of Brassica juncea (L.)  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 1-2 2012, Pages: 35-40

The present experiment was focused to study the effect of different concentrations of NaCl on shoot multiplication in Brassica juncea. In vitro culture was established on MS medium supplemented with 1.0 mg/l BAP. NaCl at various concentrations was ad...

Rapid Green Synthesis of Silver Nanoparticles Using Seed Extracts of Foeniculum vulgare and Screening of its Antibacterial Activity  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 1-2 2012, Pages: 29-34

In the present investigation we report a green chemistry approach for the biological synthesis of silver nanoparticles using the seed extract of Foeniculum vulgare Mill (sompu). The synthesized nanoparticles were structurally characterized by UV-VIS ...

In Vitro Studies and RAPD analysis in Hybanthus enneaspermus (L.) F. Muell. - A Medicinal Plant  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 1-2 2012, Pages: 47-54

Random amplified polymorphic DNA (RAPD) markers were used to evaluate the genetic stability of micropropagated plants of Hybanthus enneaspermus(L.) F. Muell. Ten arbitrary decamers were used to amplify DNA from in vivo and in vitro plant material to ...

Induction of In Vitro Roots from Leaf Callus of Ocimum basilicum L. and O. tenuiflorum L.  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 1-2 2012, Pages: 1-14

In vitro roots developed from leaf callus of Ocimum basilicum L. and Ocimum tenuiflorum L. is described in this study. Murashige and Skoog (MS) medium supplemented with different concentrations (0.25-2.5 mg/l) of 2,4-D alone and in combination with N...

Tissue Culture and Genetic Transformation of Groundnut or Peanut (Arachis hypogaea L.)-A Review on Problems and Progress  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 1-2 2012, Pages: 1-14

Groundnut or Peanut (Arachis hypogaea L.) is economically the most important species of the legume family. Peanut is an important oilseed and cash crop in warm tropical and sub tropical regions throughout the world. It is cultivated for its edible se...

In vitro Regeneration of Alpinia galanga - An Important Medicinal Plant  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 12, Issue: 1-4 2011, Pages: 91-94

Alpinia galanga comonly known as greater galangal is a tall, perennial, rhizomatous herb belongs to Zingiberaceae family. It is widely used in traditional system of medicine and it is facing threat of extinction owing to indiscriminate and unsustaina...

Micropropagation of Pedalium murex L. Through Shoot Tip Explant Culture  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 12, Issue: 1-4 2011, Pages: 87-90

In vitro regeneration was developed from shoot tip explants of Pedalium murex. MS medium supplemented with different concentration of BAP (2 mg/l - 8mg/l) and IAA (2 mg/l) respectively were tested for their efficiency multiple shoots induction. The h...

Mass Propagation of Spilanthes calva L. Through Shoot tip Explants  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 12, Issue: 1-4 2011, Pages: 83-86

Mass Propagation of Spilanthes calva .L was achieved through shoot tip explants. The explants were cultured on MS medium supplemented with BAP (4.44-22.20 �M) in combination with NAA (2.69 �M ). The rate of shoot proliferation was very high...

Preparation and Characterization of Silver Nanoparticles Impregnated With Chitosan Scaffolds  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 12, Issue: 1-4 2011, Pages: 77-82

We report here, the biosynthesis of silver nanoparticles embedded in chitosan. Prawn shells were treated with 5% sodium hydroxide and 5N HCl to remove protein and calcium, respectively. To convert chitin present in the shells into chitosan, they were...

DNA Marker Assisted Incorporation of Lr35 Gene in Wheat  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 12, Issue: 1-4 2011, Pages: 71-76

There are more than fifty leaf rust resistance (Lr) genes which can provide resistance against specific leaf rust pathotypes. One such gene Lr35, an adult plant resistance gene, has remained effective under Indian conditions. To utilize this gene in ...

Induction of Multiple Shoots from Shoot Tip Explants of Cucumber (Cucumis sativus L.)  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 12, Issue: 1-4 2011, Pages: 63-70

The present study is focused on induction of multiple shoots from shoot tip explants of cucumber. Shoot tip explants were excised from 11-day-old seedlings and cultured on MS medium fortified with different concentrations of BAP alone (0.5-2.5 mg/l) ...