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In Vitro Plant Regeneration from Different Explants of Sorghum (Sorghum bicolor L. Moench)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 3-4 2005, Pages: 101-108

An efficient plant regeneration system was developed for sorghum from different explants taken from germinating seedlings of three different varieties. The leaf base, the mesocotyl and macerated shoot apex were placed on Linsmaier and skoog(LS) mediu...

Selection in Early Breeding Generations for Leaf Rust Resistance Genes in Wheat (Triticum aestivum) with Molecular Markers

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 3-4 2005, Pages: 95-100

Selection of plants based on DNA marker linkage before the phenotypic trait for resistance is expressed, holds promise for greatly accelerating the rate of development of disease resistant crop plants. The presence of rust resistance genes Lr10, Lr26...

Encapsulation and Regeneration of Aseptic Shoot Buds of Turmeric (Curcuma longa L.) 

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 3-4 2005, Pages: 89-94

In vitro non-embryonic vegetative propagule shoot buds excised from the vegetative bud of turmeric variety Suguna (Curcuma longa L.) cultured on LSBM supplemented with BAP (4 mgl-1 ) were encapsulated in two types of hydrogels such as Sodium alginate...

Studies on Induction of Hairy Roots in some Leguminous Crops with Wild Strains of Agrobacterium Rhizogenes and their Morphogenic Response ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 3-4 2005, Pages: 81-88

The Susceptibilty of seven different leguminous crops such as Arachis hypogeae, Cajanus cajan, Cicer arietinum, Glycine max, Vigna aconitifolia, Vigno mungo, and Vigno radiata to three different wild type Agrobacterium rhizogenes strains- A4,LBA 9402...

In Vitro Storage of Gladiolus: Use of Growth Retardants and Osmotica

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005,

The effect of alar, maleic hydrazide and mannitol on growth and storability of micro-shoots of gladiolus was assessed. Alar (B-9) at a concentration of 40 mg per litre was best in extending the storability of cultures with the least shoot length (7.2...

In Vitro Shoot Multiplication in Ocimum basilicum L.

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 73-76

Profuse callus initiation was obtained from internode and leaf explants inoculated on MS medium supplemented with 1 mg/l 2,4-D. Indirect organogenesis was observed from the leaf and internode explants inoculated on MS medium supplemented with 0.3-0.5...

In Vivo Regeneration of Apical Bud Syn Seeds of Mulberry (Morus indica L.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 69-72

Syn seed were successfully produced by encapsulating non-embryogenic propagule apical buds excised from aspetic shoots of mulberry varirty-S54 (Morus indica L.). The syn seed were sown under non-aspetic condition in petridish containing different com...

Plantlet Regeneration through Multiple Shoot Induction in Cajanus cajan (L.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 65-68

A simple protocol was established for plantlet development through multiple shoot induction, elongation and their rooting from the mature seed explants of Cajanus cajan (L.) Millspaugh cv.ICPL93115. Murashige and skoog (1962) medium supplemented with...

In Vitro Germination of Hevea Somatic Embryos : Effect of Cytokinins

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 61-64

A successful procedure for somatic embryo germination and plant regeneration from immature anther derived callus of RRII 105, a high yield clone of Hevea brasiliensis, has been described. Of the four cytokinins (BA, ZEA, KIN, TDZ) tested, TDZ at 0.25...

Cost Effective In Vitro Conservation of the Threatened Malabar Daffodil Orchid using Protocorm-Like Bodies

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 57-60

Cost effective contamination free two-step protocol for in vitro conservation of Ipsea malabarica (Reichb. f) J.D.Hook., �The Malabar Daffodil Orchid� an endemic and endangered orchid of the Western Ghats of Kerela using protocorm-like bodi...

In Vitro Regeneration of Centella asiatica L.

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 53-56

Tissue culture technique has been established as a useful ex-situ conservation strategy for endangered species. This paper describes in vitro regeneration of Centella asiatica L. (Urb.) (Apiacae) a threatened species and preliminary estimation of sec...

In Vitro Somatic Embryogenesis from Seedling Explants of Bean (Phaseolus vulgaris L.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 47-52

A rapid highly repetitive and efficient protocol was developed for inducing indirect somatic embryogenesis using seedling explants of bean (Phaseolus vulgaris L.) viz., immature cotyledon, mature cotyledon, stem and leaf segments. Explants were cultu...

An Efficient Protocol for Organogenic Callus Induction and Plant Regeneration in Tomato (Lycopersion esculentum Mill.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 41-46

Organogenic callus induction and plant regeneration was achieved by using hypocotyls, leaf and cotyledonary segments. From the obtained different forms of callus, the green compact and nodular callus only produced the multiple shoots. More amount of ...

In Vitro Plant Regeneration from Young Capitulum Explants of Gerbera jamesonii

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 35-40

An efficient protocol was developed for large-scale propagation using young capitulum explants in Gerbera jamesonii. Murashige and Skoog medium supplemented with 6- Benzylaminopurine at 7 mg/l in combination with 0.1mg/l indole-3-acetic acid was foun...

Effect of Various Factors on Plantlet Regeration of Curculigo orchioides Gaertn.

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 29-34

Curculigo orchiodes Gaertn. is an endangered anticarcinogenic and aphrodisiac herb. It is available during approximately four months of the monsoon season each year. Due to over exploitation of the plant for various medicinal uses, there is a tremend...

Agrobacterium-mediated Genetic Transformation and Regeneration of Transgenic Plants in Eucalyptus tereticornis

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 23-28

Transgenic plants were regenerated from transformed embryogenesis tissue of Eucalyptus tereticornis Sm. Friable nodular callus initiated from mature zygotic embryos on MS agar medium supplemented with 2mg/l NAA were transferred to MS medium containin...

Plant Regeneration and In Vitro Flowering from Leaf and Nodal Explants of Solanum nigrum (L.) - An Important Medicinal Plant ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 17-22

Direct Organogenesis and in vitro flowering was obtained in Solanum nigrum (L.). High frequency and maximum number of multiple shoots were obtained from leaf and nodal explants on MS-medium supplemented with BAP and IAA (2.0mg/l+1.0mg/l). Regenerates...

High Frequency Shoot Regeneration and Agrobacterium � Mediated DNA transfer in Red Chilli (Capsicum annuum L.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 9-16

Different explants of three cultivars of Capsicum annuum (K1,K2 and PLR 1) were tested for their regeneration response. Shoot tip explants were co-cultivated with Agrobacterium strain EHA 105 carrying a T-DNA with CaMV 35S promoter- gus marker gene f...

AFLP Fingerprinting of some Elite Indian Cotton Genotypes

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 1-2 2005, Pages: 1-8

Amplified Fragment Length Polymorphism (AFLP) analysis was used to evaluate the genetic diversity of some elite Indian cotton genotypes. Twenty cotton genotypes belonging to Gossypium hirsutum L., and three to G. arboreum L. were analyzed with nine A...

In Vitro Regeneration of Plants from Cotyledons of Crotalaria longipes Wight & Arn.- An Endangered Legume ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 173-176

Crotalaria longipes, an endangered plant threatened with extinction was regenerated from callus of cotyledonary explants of in vitro germinated seeds. Sulfuric acid-scarified seeds produced 96% germination in Murashige and Skoog (MS) (1962) basal med...