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Effect of Colchicine on Callus Induction from Isolated Microspores in Hevea brasiliensis  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 1-2 2007, Pages: 89-92

A method for the induction of haploid callus from isolated microspores of Hevea brasiliensis has been developed. The growth promoting effects of colchicine on callus formation was proved. Anthers at the late uni-nucleate to the early bi-nucleate stag...

Callus Induction and Plant Regeneration in Sesame (Sesamum indicum L.)  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 1-2 2007, Pages: 85-88

Callus induction through hypocotyls or sub-hypocotyls or cotyledonary explants is routine in sesame (Sesamum indicum L.) where the explants are harvested after 8-10 days and were transferred on to callus induction media separately where callus prolif...

In Vitro Shoot Regeneration from Water Stress Tolerant Callus Culture of Tomato (Lycopersicon esculentum Mill.)  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 1-2 2007, Pages: 79-84

A protocol was developed for in vitro shoot regeneration from water stress tolerant callus culture of tomato cv. solan vajar on MS medium supplemented with different concentrations and combinations of various auxins and cytokinins along with 100 g/l ...

Rhizofiltration of Cadmium and Lead by Hairy Root Cultures of Brassica juncea L. and the Influence of the Metals on Antioxidative Enzymes  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 1-2 2007, Pages: 73-78

Plant hairy roots induced by Agrobacterium rhizogenes can be used as a model system to study the uptake of heavy metals and the influence of metals on the antioxidative enzymes. Hairy roots induced in Brassica juncea L. Czern and Coss. when used for ...

In Vitro Propagation of Pergularia daemia (Forsk.) Chiov.  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 1-2 2007, Pages: 67-72

The present study was conducted to establish a protocol for rapid propagation of Pergularia daemia (Asclepiadaceae) through different aseptic seedling explants. 40 days old aseptic seedlings grown on half strength MS medium were used as explant. All ...

In Vitro Regeneration of Plantlets and Micro Tubers from Nodal Segments of Dioscorea hispida (Dennst.)   ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 1-2 2007, Pages: 61-66

Dioscorea hispida is a climber that produces starch rich subterranean tubers that yield delicious Baichandi on processing. Nodal pieces of climber produced plantlets and micro tubers in vitro. Explants from greenhouse plants were established on MS me...

Role of Age and Orientation of Cotyledons Explants on Multiple Shoot Production in Chickpea (Cicer arietinum L.)  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 1-2 2007, Pages: 53-60

Two-day-old seedling explants of chickpea (Cicer arietinum L. cv. BG-256) when placed with their micropylar end touching the medium (orientation c) in modified Murashige and Skoog's medium containing 5 �M of BAP showed maximum response in terms ...

Factors Enhancing Agrobacterium tumefaciens mediated Gene Transfer in Chilli (Capsicum annum L.)  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 1-2 2007, Pages: 47-52

A reliable and highly efficient system of transforming shoot tips mediated by Agrobacterium tumefaciens was developed in Capsicum. Various aspects of transformation were examined in efforts to improve the efficiency of producing transformants. Agroba...

In Vitro Conservation of Plumbago indica - A Rare Medicinal Plant  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 1-2 2007, Pages: 39-46

Present work has been conducted on in vitro conservation of a rare medicinal plant, Plumbago indica, through storage of germplasms derived from micropropagated progenies of the species. Direct regeneration of axillary shoots and their multiplication ...

In vitro Propagation of Elite Neem (Azadirachta indica A. Juss) of Gangetic Plain with High Azadirachtin Content  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 1-2 2007, Pages: 33-38

In vitro propagation protocol of an elite Neem (Azadirachta indica A. Juss) plant having high Azadirachtin content has been standardized. Elite Neem was identified after resource survey analysis of plants from six districts of West Bengal having thre...

Enhanced Production of Psoralen, A Furanocoumarin in Callus Cultures of Cullen corylifolium (L.) Medik.  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 1-2 2007, Pages: 27-32

Callus cultures derived from internodes and leaf explants of a medicinally important plant, Cullen corylifolium were established on Z medium fortified with 9.04 �M 2,4-D and 9.3 �M kinetin. Psoralen, a furanocoumarin, was isolated from call...

In Vitro Callussing and Xylogenesis of Allium sativum L.  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 1-2 2007, Pages: 19-26

Callus culture for Allium sativum L. was established from shoot buds dissected out from the cores of cloves. Callus was induced through inoculation of explants in MS basal media supplemented with 2,4-D, 1 mg l-1 . The callus was maintained through su...

Advances in Genetic Transformation of Leguminous Crops  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 8, Issue: 1-2 2007, Pages: 1-18

Crop legumes play a pivotal role as the major source of protein in the diet of the population of developing countries, while some crop legumes like peanut and soybean also serve as a source of high quality oil. Although several transgenic crops have ...

In Vitro Seed Germination of Pecteilis Gigantea: A Rare and Endangered Orchid from Western Ghats  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 191-194

Tiny seeds of Pecteilis gigantea were cultured on different nutrient media such as MSBM, VWBM, KCBM, BGBM and FBBM supplemented with CM (15%) to study their germination frequency. The germinated seeds (96%) formed protocorm like bodies (PLB�s) o...

Histological and Biochemical Characterization of the in vitro Cultures of Zamia furfuracea L.  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 187-190

Cycads are difficult to culture. Lignification of cells in the culture and early differentiation of xylem in cultures of Zamia furfuracea results in the failure of differentiation. The failure in morphogenesis and regeneration in cycads is due to the...

Production of Plantlets from Encapsulated In Vitro Shoot Buds and Somatic Embryos of Agave vera-Cruz Mill.  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 183-186

In vitro shoot buds and somatic embryos from organogenic and embryogenic calli respectively derived from shoot tip cultures of Agave vera-cruz were isolated and encapsulated with sodium alginate and dihydrated calcium chloride. These alginate beads w...

Tissue Culture Studies for Shoot Multiplication and Herbicide Tolerance in Certain Grain Legumes  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 179-182

Plant tissue culture and Biotechnology is considered in a wide sense which comprises the various culture methods of plant organs and explants to facilitate experimental approaches with a large objective of developmental biology in grain legumes for c...

Somatic Embryogenesis from Nucellar Tissues of Mango (Mangifera indica Linn.)  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 175-178

Callus induction and somatic embryogenesis were obtained from de-embryonated nucellar tissues of young mango (Mangifera indica Linn.) cultivar �Natumanga� fruits on MS medium supplemented with 4.52 �M 2,4-D. Mostly globular/heart shape...

Effect of Azospirillum on In Vitro Regenerated Bananas  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 171-174

Considering the current importance attributed to organic products, the present study was designed to evaluate the effect of Azospirillum, NPK fertilizer and their combinations on six categories of plants in bananas namely, nendran and poovan shoot ti...

Callogenesis of Sida rhombifolia L.: A Threatened Medicinal Plant  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 167-170

Protocol for callogenesis of a species belonging to the genus Sida (Malvaceae) has been standardized. Sida rhombifolia L. var. rhomboidea (Roxb.) Mast., the 'swetberela', is an important medicinal plant used in Rural Bengal. The plant is presently no...