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HIGH FREQUENCY PLANT REGENERATION IN WITHANIA SOMNIFERA (L.) DUNAL  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 14, Issue: 3-4 2013, Pages: 139-146

At 1.5 mg/l BA, 78% of nodal explants produced shoots from the pre-existing axillary meristems (PEM) (4 shoots/node) within 6 weeks. A comparatively higher number of shoots were produced (11 shoots/node) at 1.5 mg/l BA with 0.3 mg/l IAA combination a...

EFFECT OF AUXINS AND CYTOKININS ON SHOOT FORMATION OF GERBERA JAMESONII  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 14, Issue: 3-4 2013, Pages: 128-138

Somatic tissues and seeds were tried for raising reproducible cultures in Gerbera jamesonii. Mature embryos and leaf sections were used as explants sources and cultured on MS medium supplemented with various auxins and cytokinins in varying concentra...

GERMPLASM EVALUATION IN BAMBOOS: FROM CHROMOSOMES TO MOLECULAR MARKERS  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 14, Issue: 3-4 2013, Pages: 115-127

Bamboo, an evergreen, perennial, eco-friendly and multipurpose plant, is one of the most important non-timber forest product. About two billion depend economically on bamboo, and international trade in bamboo amounts to over 2.5 billion US dollars pe...

HIGH FREQUENCY SHOOT REGENERATION OF HELIOTROPIUM ZEYLANICUM (BURM.F.) LAM. FROM NODAL EXPLANT  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 14, Issue: 3-4 2013, Pages: 111-114

An efficient protocol was developed for mass propagation of Helioropium zeylanicum from nodal explant in this present study. Nodal segment explants were inoculated on Murashiqe and skoog (1962) basal medium containing 0.8% agar, 3.0% sucrose and diff...

EFFICIENT PLANTLET REGENERATION FROM CULTURED NODAL SEGMENT OF WITHANIA SOMNIFERA (L.) DUNAL  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 14, Issue: 3-4 2013, Pages: 99-110

Nodal segment explants of two genotypes of Withania somnifera viz: JA-20 and MWS-100 were cultured on MS basal media fortified with different concentrations and combinations of diverse auxins and cytokinins as sole as well as in combinations. Conside...

CHROMIUM HEAVY METAL PHYTOTOXICITY INDUCED PHYSIOLOGICAL AND BIOCHEMICAL CHANGES IN SORGHUM SEEDLINGS GROWN UNDER IN VITRO  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 14, Issue: 3-4 2013, Pages: 90-98

Chromium is a heavy metal; this element is considered as an environmental hazard. Toxicity effects of chromium on growth and development of plants including inhibition of germination process decrease of growth and biomass of plant. The present study...

APPLICATION OF RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS TO DETECT THE GENOTOXIC EFFECT OF MERCURY (HG) HEAVY METAL STRESS ON MENTHA ARVENVSIS SEEDLINGS  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 14, Issue: 3-4 2013, Pages: 84-89

At present, several plant species have been used as bioindicators, and recent advances in molecular biology have led to the development of a number of molecular marker assays for DNA variation analysis in ecogenotoxicology. In the present study, Ment...

IN VITRO MICROPROPAGATION OF PLUMBAGO ZEYLANICA LINN. THROUGH NODALSEGMENT AND LEAF EXPLANTS  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 14, Issue: 3-4 2013, Pages: 72-83

Nodal segment and leaf explants of Plumbago zeylanica were cultured on three basal media fortified with different concentrations and combinations of diverse auxins and cytokinins as sole as well as in combinations. For cultured nodal segment, culture...

TISSUE CULTURE STUDIES ON SARACA ASOCA (ROXB.) DE WILDE: A VULNERABLE MEDICINAL PLANT FROM WESTERN GHATS  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 14, Issue: 1-2 2013, Pages: 66-71

Saraca asoca (Roxb.) de Wilde (Ceasalpiniaceae) is a vulnerable medicinal as well as ornamental potential tree, distributed in the different parts of Western Ghats. Bark of S. asoca is extremely useful in uterine affections especially menorrhagia due...

MICROPROPAGATION OF ALPINIA OFFICINARUM: AN ANTI-CANCER MEDICINAL PLANT  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 14, Issue: 1-2 2013, Pages: 60-65

Alpinia officinarum (lesser galangal) belonging to the family Zingiberaceae is an important aromatic perennial herb distributed widely in India, China and Asia. Alpinia officinarum is widely used in the pharmaceutical industry for cancer therapeutic ...

ESTABLISHMENT OF LOW- COST EFFECTIVE PROTOCOL FOR MASSIVE IN VITRO PROPAGATION IN POLIANTHES TUBEROSA LINN  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 14, Issue: 1-2 2013, Pages: 49-59

To search out the responsive culture medium combination with higher regeneration potential, stem disc of Polianthes tuberosa was cultured on MS basal medium fortified with different auxins and cytokinins in varying concentrations and combinations. Cu...

CELLULAR AND MOLECULAR MECHANISMS OF HEAVY METAL TOLERANCE IN PLANTS: A BRIEF OVERVIEW OF TRANSGENIC PLANTS OVER-EXPRESSING PHYTOCHELATIN SYNTHASE AND METALLOTHIONEIN GENES  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 14, Issue: 1-2 2013, Pages: 33-48

Heavy metalsare important environmental pollutants and many of them are toxic even at very low concentrations. Pollution of the biosphere with heavy metals has accelerated dramatically since the industrial revolution. Metal toxicity is one of the maj...

STUDIES ON IN VITRO MORPHOGENESIS FROM BULB SCALE AND LEAF DISC EXPLANT OF AMARYLLIS BELLADONNA L  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 14, Issue: 1-2 2013, Pages: 22-32

An efficient method for rapid propagation of Amaryllis belladonna L. has been developed in this study. Bulb scales and leaf discs were used as explants and cultured on MS medium amended with diverse auxins and cytokinins in varying concentrations as ...

PLANT REGENERATION FROM EXPLANTS OF DELONIXELATA L. VIA ORGANOGENESIS  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 14, Issue: 1-2 2013, Pages: 12-21

In this paper we report the establishment of Delonix elata regeneration through organogenesis. We used node old tree, seedlings shoot tip, leaf and stem explants excised from, Murashige and Skoog (MS) medium supplemented with various concentrations o...

MICROCLONING STUDIES IN ALOE BARBADENSIS  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 14, Issue: 1-2 2013, Pages: 1-11

Stem disc explant of Aloe was cultured on MS basal medium containing different auxins, cytokinins as sole as well as in combinations. Culture medium MS2D (MS+2.0 mg.l-1 2,4-D) initiated callus in higher proportion. Proliferation medium MS3B (MS+3.0 m...

Plant Regeneration and In Vitro Flowering from Nodal Explants of Hydrolea zeylanica - A Rare Medicinal Plant  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 3-4 2012, Pages: 147-150

An efficient micropropagation and in vitro flowering protocol was developed for an important and rare medicinal plant Hydrolea zeylanica. Multiple shoots regeneration from nodal explants was achieved on MS medium containing different concentration of...

Biochemical analysis between In Vivo and In Vitro Regenerated Plant Leaves of Solanum nigrum L.  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 3-4 2012, Pages: 141-146

To compare the efficacy for biochemical analysis of in vitro propagated plants and field grown plants of Solanum nigrum L. Maximum shoot multiplication were observed MS medium containing 0.75 mg/l BAP+0.5 mg/l IBA and 0.1 mg/l NAA. Immature tissues s...

Production of Aristolochic Acid (AA) I and II from Aristolochia indica and Aristolochia bracteolate through In Vitro Culture  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 3-4 2012, Pages: 131-140

Callus of Aristolochia indica and Aristolochia bracteolata was induced on MS basal medium supplemented with 3.0 mg/l and 1.0 mg/l of NAA from leaf explants of A. bracteolata and A. indica respectively. Aristolochic acid (AA) I and II were identified ...

In vitro Plant Regeneration via Direct Organogenesis from Shoot Tip Explants of Cucumber (Cucumis sativus L. Green long)  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 3-4 2012, Pages: 125-130

The present study is focused on induction of multiple shoots from shoot tip explants of cucumber. Shoot tip explants were excised from 7-day-old seedlings and cultured on MS medium fortified with different concentrations of (0.5-2.5 mg/l) BAP alone a...

Isolation, Characterization and Detection of Cry Gene Contents from South Indian Isolates of Bacillus thuringiensi  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 13, Issue: 3-4 2012, Pages: 111-124

Bacillus thuringiensis Berliner based bio pesticides have been successfully used worldwide for the control of agricultural pests and vectors of human diseases; constitutes a large family of bacterial subspecies highly specialized as insect pathogens ...