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Proteomics: A Post Genomic Revolution

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 2, Issue: 3-4 2001, Pages: 71-88

Proteomics provide a way of characterizing biological processes and is an important adjunct to micro-array approaches and DNA technology. Proteomic techniques, unlike traditional methods, can collect data for many proteins at once, adding rapidly to ...

In Vitro Flowering of Vitex negundo L. A Medicinal Plant

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 2, Issue: 1-2 2001, Pages: 67-70

In vitro plant regeneration and flowering were achieved from two explants of Vitex negundo L on MS medium containing different concentrations of auxins and cytokinins. The highest percentage (95.3%) of flowering was noticed from nodal explants cultur...

Molecular Characterization of Hairy and Fibre Characters in Cotton (Gossypium hirsutum L.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 2, Issue: 1-2 2001, Pages: 63-66

We report here the molecular confirmation of a RFLP marker closely linked to genes affecting hairiness in cotton. Among 10 DNA probes used, A1152 probe was hybridised with XbaI digested cotton genomic DNA at chromosome number 6 for the genes affectin...

In Vitro Micropropagation of Dictyospermum ovalifolium Wight. A Rare and Endemic Plant in Western Ghats, India ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 2, Issue: 1-2 2001, Pages: 57-62

Tissue culture techniques have been established as a useful approach for ex-situ conservation of rare, endemic or threatened plant species. This paper describes the micropropagation of Dictyospermum ovalifolium (Commelinaceae) an endangered plant spe...

In Vitro Plant Regeneration in Pigeon Pea [Cajanus cajan (l.) Millsp] via Organogenesis

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 2, Issue: 1-2 2001, Pages: 49-56

Seedling explants such as cotyledons, leaves and stems from two cultivars of Pigeon pea were assessed for callus induction and shoot bud differentiation. The frequency of callus initiation from cotyledons, leaves and stems was 90, 80 and 85% respecti...

Detection of Sandal Spike Phytoplasma by Immunomicroscopy

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 2, Issue: 1-2 2001, Pages: 41-48

Sandal tissues affected by spike disease were screened for the presence of sandal spike phytoplasma using the immunomicroscopic techniques immunofluorescence microscopy and immunoelectron microscopy. The tests were found to be highly sensitive to det...

In Vitro Callus Induction and Plantlet Regeneration from Young Inflorescence and Leaf Explants of Pearl Millet

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 2, Issue: 1-2 2001, Pages: 33-40

In vitro response of young inflorescence and leaf explants from three genotypes viz., hybrid cumbu (X6), medium duration inbred (PT 1479) and high yielding inbred (PT 4671) of pearlmillet was described. The basal medium used for callus induction and ...

Isolation and Characterization of a Serine Proteinase Inhibitor cDNA from Cabbage and its Antibiosis in Transgenic Tobacco Plants

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 2, Issue: 1-2 2001, Pages: 19-32

Plant proteinase inhibitors (PIs) are of special interest because of their role in plant defense against herbivorous insects. We isolated a cDNA clone for a serine PI from Brassica oleracea, cabbage (BoPI). A comparison of the putative coding sequenc...

Biotechnological Applications in Grain Legume Improvement: A Critical Review

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 2, Issue: 1-2 2001, Pages: 1-12

Tissue culture and genetic transformation play a vital role in the improvement of cultivated plants for disease and insect resistance, beneficial traits improvement etc. Numerous studies describe different media and hormone combinations for eliciting...

Altered Segregation Ratio in Anther Culture derived Plant Progenies Generated from F1 Hybrids of Brassica juncea

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 1, Issue: 3-4 2000, Pages: 115-121

Microspore derived plant population was raised using anthers from F1 hybrid plants obtained by a reciprocal cross between two cultivars of Brassica juncea (cv. RM-198 and cv. RJ-15). The F2 population segregation ratio and frequency distribution of t...

In vitro Plant Regeneeration from Immature Leaflet-let derived Callus Cultures of Cicer arietinum L. via Organogenesis ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 1, Issue: 3-4 2000, Pages: 109-115

Immature leaflet explants of Cicer arietinum L. cv Pusa-256 developed organogenic calli on MS basal medium supplemented with 2,4-D (1-25 �M) and BAP (10 �M), such calli on transfer to 5, 10 �M of BAP and TDZ along with 0.1 �M IBA ...

Callus Induction and Regeneration of Plantlets from Seed Explants of Pearl Millet [Pennisetum glaucum (L.)R. Br.]

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 1, Issue: 3-4 2000, Pages: 101-108

In vitro response of eight pearl millet genotypes was evaluated in the present study. When the seed was used as initial explants, high percentage (87%) of callus induction was noticed in the genotype X6 followed by PT 4671 (73%) and PT 1479 (68%) wit...

High Frequency Plantlet Regeneration from Hypocotyl Explants of Tomato (Lycopersicon esculentum Mill.) via Organogenesis

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 1, Issue: 3-4 2000, Pages: 95-100

A protocol was developed for high frequency plant regeneration from hypocotyls explants of Lycopersicon esculentumon MS medium supplemented with different concentrations and combinations of various auxins and cytokinins. Frequency of callus initiatio...

Biochemical Characterization of Near-Isogenic Wheat Lines Carrying Various Rust Resistance Genes

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 1, Issue: 3-4 2000, Pages: 87-94

Near-isogenic wheat lines carrying various rust resistance genes were analysed for changes in various biochemical parameters. Rust resistant near-isogenic wheat lines had higher lipid content and retained relatively steady level of chlorophyll conten...

Immunodetection of Teliospores of Karnal Bunt (Tilletia indica) of Wheat using Fluorescent Staining Tests

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 1, Issue: 3-4 2000, Pages: 81-86

Polyclonal antibodies were raised against intact teliospores in Newzealand white rabbits for the immunodetection of karnal bunt (KB) teliospores.The indirect immunofluoresence (IIF) test was developed using anti-teliospores serum and binding was moni...

Biotechnology: A Promising Tool for Conservation of Phytodiversity

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 1, Issue: 3-4 2000, Pages: 73-80

Biological diversity is the wealth of life on earth, the millions of plants, animals and microorganisms, the genes they contain, and the intricate ecosystems they help build into the living environment. Biological diversity is the end result of four ...

Edible Vaccines: An Overview

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 1, Issue: 3-4 2000, Pages: 67-72

Plants are important renewable resource commodities and transgenic plants expressing foreign protein of industrial or pharmaceutical use represent an economical alternative to fermentation-based production systems. There is an increasing demand for c...

Identification of a Molecular Marker Linked to a Gene Conferring Resistance to Indian Pathotype- I Of Xanthomonas oryzae Pv Oryzae in Rice

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 1, Issue: 1-2 2000, Pages: 63-66

Bacterial Blight (BB) of rice is a major production constraint to rice farming in India. The feasibility of identifying molecular marker(s) linked to a bacterial blight resistance gene from a rice variety Ajaya. Which is non-allelic to other known re...

Efficient Callus Induction and Plant Regeneration from Immature Anther of Rice (Oryza sativa L.) via Somatic Embryogenesis

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 1, Issue: 1-2 2000, Pages: 55-62

High frequency somatic embroygenesis and plantlet regeneration from immature anther cultures of rice was described. Immature anthers were cultured on N6 medium with different concentrations of 2,4-D along with 1.0mg/l BAP and produced embryogenic cal...

In Vitro Plant Regeneration and Recovery of Cowpea (Vigna unguiculata) Transformants Via Agrobacterium-mediated Transformation ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 1, Issue: 1-2 2000, Pages: 47-54

In vitro regeneration of plants from mature seed-derived shoot apices and embryonal axes and their competence to Agrobacterium-mediated genetic transformation have been assessed in two cultivars of Vigna unguiculata. The explants produced multiple sh...