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Multiple Shoot Induction in Capsicum annuum L. Cv. Early California Wonder

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 95-100

A rapid and reproducible regeneration protocol has been established in Bell pepper (Capsicum annuum L. cv. Early California wonder) via in vitro shoot multiplication. Multiple shoots were induced from shoot tip explants cultured on MS medium fortifi ...

Multiple Shoot Induction and Plant Regeneration of Peanut (Arachis hypogaea L.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 3-4 2004, Pages: 89-94

This study was designed to standardize the effect of picloram on plant regeneration from cotyledon explants of groundnut. High frequency of callus formation was noticed when the explants was cultured on MS medium with picloram. Three-week-old calli w...

In Vitro Plant Regeneration from Shoot Meristem Explants of Wheat (Triticum aestivum L.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 85-88

A simple procedure for high frequency multiple shoot regeneration from shoot meristem of wheat (Triticum aestivum L.) has been developed. Shoot apices from 1-week-old aseptically grown seedlings were cultured in vitro on MS medium supplemented with e...

Assessment of Genetic Diversity and Population Discrimination in Hypericum hookerianum of Western Ghats using RAPD

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 81-84

The genetic diversity in Hypericum hookerianu collectd from six different sites in Western Ghats was assessed. The aim was to compare inter and intra population genetic distances and to find out the areas of maximum genetic diversity. Out of 40 prime...

In Vitro Plant Regeneration from Stem Explants of Phaseolus vulgaris L

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 77-80

In vitro regeneration of shoot buds and roots from stem explants of the French bean, isolated from 5-6-day-old seedlings was studied under defined cultural conditions. Stem explants were cultured on MS medium supplemented with various concentrations ...

In Vitro Shoot Multiplication in Rosa indica L.

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 73-76

A rapid and reproducible regeneration protocol has been established in Rosa indica via nodal shoot multiplication. Nodes of 0.5 cm long cultured on MS medium supplemented with IAA and BA produced multiple shoots. After 30 days the shoots began yellow...

Interspecific Hybridization in genus Helianthus through Embryo Rescue

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 65-72

In a direct approach to rescue interspecific hybrids, immature embryos of cv. Morden were cultured at different developmental stages i.e. 1-4 weeks after fertilization on four percentage. Maximum germination percentage was observed from 4-weeks-old e...

Study of Androgenesis using Morphometrics and Regeneration in Indian Mustard (Brassica juncea Czern. and Coss.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 59-64

Anther culture involves identification, isolation and culture of appropriate stage of microspore, which world induce callus /embryo and subsequently give rise to androgenic plant. Anther culture of yellow seeded mustard (Brassica juncea) was carried ...

Effect of Fungicides and Antibiotics to control Microbial Contamination in Hevea Cultures

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 51-58

In vitro toxicity and microbial contamination control efficiency of three fungicides- benomyl, bavistin and fytolan and three antibiotics-nystatin, amphoterincin-B and streptomycin were assessed by incorporating them in the Hevea. shoot culture mediu...

Influence of the Physiological Age and Position of the Nodal Explants on Micropropagation of Field-Grown Dendrocalamus strictus Nees ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 45-50

An efficient and reproducible protocol for plant regeneration through in vitro culture of Dendrocalamus strictus is reported. Murashige and Skoog (MS) basal medium supplemented with N6-benzyladenine and adenine sulphate resulted in high frequency of ...

Effect of Age of Explants on Somatic Embryogenesis and Plant Regeneration in Eucalyptus tereticornis SM

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 39-44

Plant regeneration was achieved via somatic embryogenesis in Eucalyptus tereticornis SM. Callus was induced from cotyledon explants isolated from 7-to-28 day-old seedlings at weekly intervals when cultured on MS basal medium with different concentrat...

In Vitro Regeneration of Celastrus paniculatus Willd. -A Rare Medicinal Plant

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 33-38

In vitro regeneration protocol through the stem callus culture of Celastrus paniculatus Willd was established. The explants induced callus on LS medium supplemented with 2% fructose, 6 uM Kn and 1 uM IBA. Interaction of the same hormones at lower con...

In Vitro Growth of Tomato Leaf Bit Calli under NaCl Stress

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 27-32

Leaf bit derived callus from four tomato (Lycopersicon esculentum Mil.) cultivars (CO 2, CO 3, PKM 1, UC 82) were subjected to undergo stress-using NaCl. The survival rate of the leaf bit calli at different concentrations of NaCl (0.5, 1.0, 1.5, 2.0 ...

Optimization of Micropropagation Protocol for Sugarcane (Saccharum officinarum L.) Cultivars

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 21-26

A common micropropagation protocol for eight cultivars of sugarcane (Saccharum officinarum L.) was developed. A combination of 0.5 mg l-1 each of BAP, Kin, IAA and GA3 in MS medium was found to be the best for shoot elongation from apical bud explant...

In Vitro Regeneration of Cotton Plants from Shoot Tip Explants: A Simple and Alternative Method

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 15-20

In order to develop the transgenic and disease resistant plants through in vitro mutagenesis easily regenerable embryonic tissues are required. Meristem shoot tip of normal and fertile plants of Gossypium hirsutum var. MCU 5 and MCU 11 were regenerat...

In Vitro Development and convertibility of Nucellar Embryos of Mangifera indica L. Bappakai, A Dwarfing Rootstock ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 7-14

Embryos differentiated fromm the nucellus of young ovular halves of Mangifera indica L. Bappakai, and important dwarfing rootstock variety, on BM1 medium supplemented with 0.25 mgl-1 BA and 1 mgl-1 NAA. Their proliferation was achieved in the medium ...

Effect of Genotype and Culture Medium on Callus Proliferation and Morphogenesis of Immature Embryonic Axes of Soybean (Glycine Max L. Merr.). ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 5, Issue: 1-2 2004, Pages: 1-6

Immature embryos of eleven genotypes of Glycine max were cultured on MS medium supplemented with different levels of growth hormones, viz.8.0 mg.l-1 NAA (MS8N), 2.0mg.l-1 NAA + 0.2mg.l-1 BA (MS2NB) and 3.0 mg.l-1 BA + 0.5mg.l-1 NAA (MS3BN). Highly si...

In Vitro Plant Regeneration from Nodal Explants of Syzygium cuminii L.

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 3-4 2003, Pages: 197-204

Callus induction and high frequency regeneration was obtained from nodal explants of five-year-old plants of Syzygiun cuminii when cultured on WP medium supplemented with 2,4-D (4.5 ?M) and BA (4.4 ?M). Calli were subcultured on WP medium with differ...

Agrobacterium-Mediated Genetic Transformation in Indian Spinach (Beta palonga)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 3-4 2003, Pages: 193-196

An efficient and reproducible protocol for Agrobacterium-mediated plant transformation has been standardized in Indian spinach (Beta palonga) with a standard vector (pCambia 1304). Passage wise subculture in medium containing hygromycin, the selectio...

In Vitro use of a Virus Inhibitor of Plant Origin in the Production of Carnation Latent Virus (Clv) Free Plants

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 3-4 2003, Pages: 185-188

An ecofriendly approach to tackle Carnation Latent Virus in carnation cv Scania has been standardized. Addition of four different concentrations viz. 1.0, 2.5, 5.0 and 7.5 mg/l of acetone extracts obtained from fruit and root fractions of Asparagus a...