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Direct Shoot Regeneration from Leaf Explants of Chicory (Cichorium intybus L.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 3-4 2003, Pages: 125-131

Chicory (Cichorium intybus L.) is a biennial long day plant and is economically important for its medicinal, food and ornamental values. In vitro plantlets were directly regenerated from the leaf explants in MS medium supplemented with different conc...

Rapid Micropropagation of Andrographis alata Nees. through Leaf Callus Culture

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 3-4 2003, Pages: 117-124

An efficient protocol for plantlet regeneration through the leaf calli has been established on Andrographis alata Nees. The leaf explants proliferate into luxuriant callus mass on MS medium fortified with 7 �M NAA. Interaction of the same hormon...

Plantibodies: Plants as an Alternate Production System towards Molecular Farming of Antibodies for Diverse Applications

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 3-4 2003, Pages: 103-115

Expression of antibodies in plants is one of the fields of plant genetic engineering, which was developed recently. It refers to the introduction and expression of the genes encoding antibodies or antibody fragments in plants. The most intriguing pot...

Biotechnology of Rice: Present Limitations and Future Prospects

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 1-2 2003, Pages: 95-99

Direct shoot regeneration from terminal bud and mature nodal explant were obtained in Terminalia arjuna. Nodal explants produced more shoots than terminal apex. Woody Plant medium with 2mg/BA produced maximum shoots. For rooting IAA at 1mg/l found to...

Alkaloid Production from Cell Suspension Cultures obtained from Osmotic-Stressed Callus Lines of Catharanthus roseus

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 1-2 2003, Pages: 1-12

Catharanthus roseus callus cultures established were first exposed to osmotic stress using polyethylene glycol (9.0 %, w/v)(MW 3350) in solid MS medium supplemented with 20 g/l sucrose, 2 mg/l ?-naphtalene acetic acid (NAA) and 3 mg/l 6-benzylamino p...

In Vitro Plant Regeneration and Protein Profile Analysis in Centella asiatica (Linn.) Urban

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 1-2 2003, Pages: 83-90

The lamina explants excised from in vitro raised shoots induced multiple shoots through callus phase on Murashige and Skoog (MS) medium supplemented with cytokinins alone and in combinations. Amongst all the cytokinins namely, N6-benzyladenine (BA), ...

Spontaneous Sexual Triploids from Intraspecific Crosses of Cassava (Manihot esculenta Crantz): Molecular Evidence

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 1-2 2003, Pages: 75-82

A triploid cassava with somatic chromosome number 2n=54 had been identified from a hybridisation made in 1984 involving two diploid parents, �H-165� and �Sree Sahya�. It was a first report, in cassava, at that time, and as such th...

Somatic Embryogenesis and Biochemical Analysis of In Vitro derived Plants in Mothbean (Vigna aconitifolia Jacq .)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 1-2 2003, Pages: 69-74

Efficient plant regeneration via somatic embryogenesis has been developed in Vigna acontifolia (Jacq.). Leaf explants from 10-day-old seedlings produced embryogenic callus and somatic embryos when cultured on Murashige and Skoog (MS) basal medium sup...

In Vitro Production of Capsaicin by Cell and Tissue Culture of Capsicum annuum

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 1-2 2003, Pages: 63-68

Capsaicin production by cell suspension cultures, immobilized cells, and immobilized placenta was compared with capsaicin content of different parts of fruits of Capsicum annuum at 21 days after flowering. The capsaicin content of placenta (8.73 mg g...

In Vitro Induction of Adventitious Shoot Buds and Plant Regeneration from Leaf Explants of Coffea bengalensis Roxb.: A Wild Coffee Species ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 1-2 2003, Pages: 57-62

In vitro adventitious shoot formation from foliar explants of Coffea bengalensis Roxb. was achieved. Among various combinations of growth regulators tested, N6 benzyladenine (BA) was found to be the most effective in inducing shoot buds. Shoot buds a...

High Frequency Plant Regeneration in Ashwagandha [Withania somnifera (L.) Dunal].: An Important Medicinal Plant

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 1-2 2003, Pages: 49-56

A high frequency and rapid regeneration protocol via callus and directly from various explants was developed in Withania somnifera (L.) Dunal. Callus was initiated from internodal segment, leaf, root and petiole explants on MS and B5 media supplement...

In Vitro Morphogenesis in cultivated varieties of Sorghum bicolor (L.) Moench

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 1-2 2003, Pages: 43-48

In vitro plant regeneration is achieved in cultivated varieties of Sorghum bicolor from immature inflorescence explants through embryogenic callus induction and also organogenesis. Explants were cultured on modified Murashige and Skoog's medium, supp...

Cloning and Expression analysis of Water Stress-Induced cDNAs from radicle of Indica Rice (Oryza sativa Cv. Nootripathu) by mRNA Differencial Display ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 1-2 2003, Pages: 33-42

The landraces of Indica rice (Oryza sativa L.) are well adapted to drought conditions throughout their life. Their superior germination and seedling root characters enable them to withstand drought during their early stages. To identify water stress-...

Epigenesis and High Frequency Plant Regeneration from Hypocotyl Explants of Soybean (Glycine max L. Merrill)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 1-2 2003, Pages: 23-32

High frequency somatic embryo induction was achieved in Glycine hypocotyls depending upon genotype and the explant inoculation medium. Plant regeneration efficiency was in excess of 85% following plural shoot initiation in genotype JS 90-41 when the ...

In Vitro Plant Regeneration from Nodal and Leaf Explants of Solanum surattense Burm. F. A Medicinal Plant

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 1-2 2003, Pages: 17-22

An efficient method was developed for in vitro propagation Solanum surattense Burm. f. Multiple shoot formation was noticed on Murashige and Skoog�s medium supplemented with 2 mg/l 6-benzylaminopurine and 1 mg/l kinetin from nodal explants and 4...

Enhanced Somatic Embryogenesis and Plant Regeneration in Bamboo (Dendrocalamus strictus)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 1-2 2003, Pages: 9-18

Enhanced somatic embryogenic system was developed using zygotic embryos of Dendrocalamus strictus. Linsmeir and Skoog (LS) medium supplemented with 27.14?M 2,4-D was suitable for the induction of somatic embryogenic callus. Proliferation of embryogen...

Biotechnology of Sandalwood - Current Trends and Future Needs

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 4, Issue: 1-2 2003, Pages: 1-8

Sandalwood is a commercially important aromatic tree, and is the source of fragrant heartwood and oil. Conventionally, new trees are raised through seeds and vegetative propagation has not been successful in this species. Multiplication of geneticall...

In Vitro Production of Rice Plants from Synthetic Seeds by Encapsulation of Somatic Embryos

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 155-158

Somatic embryos of rice [Oryza sativa (cv.Tellahamsa)] were encapsulated in an alginate matrix to produce synthetic seeds. Eight to ten-day-old globular somatic embryoids were found suitable for germination and plant conversion. In the present invest...

Callus Formation and Shoot Regeneration from Mature and Juvenile Explants of Murraya koenigii (Spreng)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 151-154

Murraya koenigii is a tree cultivated for its fragrant leaves used for culinary and medicinal purposes. Callus could be induced only from the internodes of mature plants. Morphogenetic callus was induced with BAP alone or in combination with NAA. The...

In Vitro Production of Virus Free Plants in Citrus reticulate

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 147-150

Virus free plantlets were successfully produced using meristem tip as explants. Meristem dome with 2 leaf primordial (Size 100-200u) was cultured in modified MS liquid media (M19) containing 4 mg l-1 Thamine, 0.01mg l-1 Riboflavin and 0.01 mg. l-1 GA...