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In vitro Regeneration of Alpinia galanga - An Important Medicinal Plant  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 12, Issue: 1-4 2011, Pages: 91-94

Alpinia galanga comonly known as greater galangal is a tall, perennial, rhizomatous herb belongs to Zingiberaceae family. It is widely used in traditional system of medicine and it is facing threat of extinction owing to indiscriminate and unsustaina...

Micropropagation of Pedalium murex L. Through Shoot Tip Explant Culture  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 12, Issue: 1-4 2011, Pages: 87-90

In vitro regeneration was developed from shoot tip explants of Pedalium murex. MS medium supplemented with different concentration of BAP (2 mg/l - 8mg/l) and IAA (2 mg/l) respectively were tested for their efficiency multiple shoots induction. The h...

Mass Propagation of Spilanthes calva L. Through Shoot tip Explants  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 12, Issue: 1-4 2011, Pages: 83-86

Mass Propagation of Spilanthes calva .L was achieved through shoot tip explants. The explants were cultured on MS medium supplemented with BAP (4.44-22.20 �M) in combination with NAA (2.69 �M ). The rate of shoot proliferation was very high...

Preparation and Characterization of Silver Nanoparticles Impregnated With Chitosan Scaffolds  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 12, Issue: 1-4 2011, Pages: 77-82

We report here, the biosynthesis of silver nanoparticles embedded in chitosan. Prawn shells were treated with 5% sodium hydroxide and 5N HCl to remove protein and calcium, respectively. To convert chitin present in the shells into chitosan, they were...

DNA Marker Assisted Incorporation of Lr35 Gene in Wheat  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 12, Issue: 1-4 2011, Pages: 71-76

There are more than fifty leaf rust resistance (Lr) genes which can provide resistance against specific leaf rust pathotypes. One such gene Lr35, an adult plant resistance gene, has remained effective under Indian conditions. To utilize this gene in ...

Induction of Multiple Shoots from Shoot Tip Explants of Cucumber (Cucumis sativus L.)  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 12, Issue: 1-4 2011, Pages: 63-70

The present study is focused on induction of multiple shoots from shoot tip explants of cucumber. Shoot tip explants were excised from 11-day-old seedlings and cultured on MS medium fortified with different concentrations of BAP alone (0.5-2.5 mg/l) ...

In vitro Propagation of Arachis hypogaea L Var. Ak 1224: Direct and Indirect Plant Regeneration  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 12, Issue: 1-4 2011, Pages: 57-62

In vitro propagation of Arachis hypogaea L through direct and indirect regeneration of shoots using various explants has been achieved. Cotyledonary nodes and cotyledonary leaves from axenic seedlings were cultured in presence of various concentratio...

Risk Assessment of Mercury Ion Heavy Metal Exposure on Physiological, Biochemical Changes and DNA Damage Using RAPD Analysis in Mentha arvensis Seedlings  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 12, Issue: 1-4 2011, Pages: 41-50

Mentha arvensis is a fast growing plant with high biomass production that is commonly growing in tropical areas. However, its ability to hyperaccumulation and tolerance to heavy metal stress has yet to be investigated. The present study is focused to...

In vitro Culture Studies on Indigofera tinctoria Linn. and Indigofera enneaphylla Linn.  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 12, Issue: 1-4 2011, Pages: 31-40

The present study was conducted to establish a protocol for direct and indirect regeneration from various explants of Indigofera tinctoria and Indigofera enneaphylla as well as to develop a well proliferating callus/suspension culture containing well...

In vitro Culture Studies on Indigofera tinctoria Linn. and Indigofera enneaphylla Linn.  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 12, Issue: 1-4 2011, Pages: 31-40

The present study was conducted to establish a protocol for direct and indirect regeneration from various explants of Indigofera tinctoria and Indigofera enneaphylla as well as to develop a well proliferating callus/suspension culture containing well...

Development of Resistant Lines against Leaf Blight Disease of Withania somnifera (L.) Dunal. Caused by Alternaria alternata through In vitro Selection  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 12, Issue: 1-4 2011, Pages: 21-30

Disease tolerant/resistant Withania (Withania somnifera (L.) cv JA-20 and MWS-100) cell lines were selected against leaf blight disease caused by Alternaria alternata. For this purpose callus and cell suspension cultures derived from mature embryos a...

In vitro Plant Regeneration via Somatic Embryogenesis for Cinnamum smophloeum Kaneh  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 12, Issue: 1-4 2011, Pages: 15-20

A protocol for somatic embryogenesis and plant regeneration from immature leaflets of Cinnamum osmophloeum has been developed. Induction of callus was on woody plant medium (WPM) supplemented with 2,4-D (2 mg/l). A high percentage of embryogenesis (6...

Biochemical and Molecular Effects of Lead Heavy Metal Toxicity in Plants: A Phytoremediation Approach  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 12, Issue: 1-4 2011, Pages: 1-14

Lead is one of the most abundant, ubiquitous toxic elements posing a critical concern to human and environmental health. It causes deleterious effects at the subcellular and molecular levels in plant cells. The effect of lead depends on the concentra...

Indirect Organogenesis of Withania somnifera - Poshita Variety.  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 11, Issue: 1-4 2010, Pages: 89-92

Withania somnifera cv. Poshita is an ethanomedicinally important plant. Seeds of the plant, sterilized and inoculated on � MS basal medium supplemented with 3% sucrose and 100 mgl-1myoinositol germinated well within three days of incubation. The...

Direct Organogenesis from Leaf Explant of Solanum Burm F.  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 11, Issue: 1-4 2010, Pages: 85-88

A rapid in vitro method for propagation of Solanum surattense Burm. F. was developed. Leaf explants were cultured on MS medium supplemented with different concentration of BAP (4.44 � 17.76 �M/l) and KIN (4.65 - 18.60 �M/l) in combinat...

In vitro shoot Multiplication in Salacia chinensis L.  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 11, Issue: 1-4 2010, Pages: 81-84

A rapid regeneration protocol has been established in Salacia chinensis via shoot tip multiplication. Shoot tips of 0.5 cm long cultured on MS medium supplemented with BA and IAA produced multiple shoots. A maximum of 13.22�0.95 shoots were prod...

Utility of Isubgol, Gum Arabic and Sago as Alternative Gelling Agents to Agar For in vitro Regeneration and Multiplication of Protocorms of Vanda testacea (Lindl.) Reichb.F. -A Rare Medicinal orchid ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 11, Issue: 1-4 2010, Pages: 75-80

Presently, the efficacy of isubgol, gum Arabic, and sago as an ecofriendly and cheap substitute to expensive gelling agent agar was tested to assess the proliferation potential of Vanda testacea protocorm segments. M medium supplemented with 2% sucro...

In vitro shoot Regeneration from Leaf Explants of Vitis vinifera (L.)  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 11, Issue: 1-4 2010, Pages: 65-74

An in vitro regeneration protocol has been developed from 5-8 mm long leaf discs excised from in vitro grown regenerants as well as one-year-old plants of three different cultivars of Vitis vinifera L viz: Thompson seedless, Karnet and Local to find ...

In vitro Multiple Shoot Regeneration from Nodal Explants of Stevia rebaudiana (Bert): An Antidiabetic Medicinal Plant  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 11, Issue: 1-4 2010, Pages: 59-64

A simple, rapid and large scale in vitro propagation method for Stevia rebaudiana has been established. Shoots were induced from nodal explants of field grown Stevia plants on MS medium fortified with different concentrations of BAP (0.5-2.0 mg/l) al...

Efficient in vitro Regeneration Protocol as an Aid to Conserve Wild Population of Centella asiatica (L.) Dunal  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 11, Issue: 1-4 2010, Pages: 51-58

In order to reduce the pressure on the wild stock of Centella, which is being exploited to meet the ever increasing demand from pharmaceutical industries, an efficient in vitro multiplication protocol has been developed. The aseptic cultures were ini...

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