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In Vitro Seed Germination of Pecteilis Gigantea: A Rare and Endangered Orchid from Western Ghats  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 191-194

Tiny seeds of Pecteilis gigantea were cultured on different nutrient media such as MSBM, VWBM, KCBM, BGBM and FBBM supplemented with CM (15%) to study their germination frequency. The germinated seeds (96%) formed protocorm like bodies (PLB�s) o...

Histological and Biochemical Characterization of the in vitro Cultures of Zamia furfuracea L.  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 187-190

Cycads are difficult to culture. Lignification of cells in the culture and early differentiation of xylem in cultures of Zamia furfuracea results in the failure of differentiation. The failure in morphogenesis and regeneration in cycads is due to the...

Production of Plantlets from Encapsulated In Vitro Shoot Buds and Somatic Embryos of Agave vera-Cruz Mill.  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 183-186

In vitro shoot buds and somatic embryos from organogenic and embryogenic calli respectively derived from shoot tip cultures of Agave vera-cruz were isolated and encapsulated with sodium alginate and dihydrated calcium chloride. These alginate beads w...

Tissue Culture Studies for Shoot Multiplication and Herbicide Tolerance in Certain Grain Legumes  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 179-182

Plant tissue culture and Biotechnology is considered in a wide sense which comprises the various culture methods of plant organs and explants to facilitate experimental approaches with a large objective of developmental biology in grain legumes for c...

Somatic Embryogenesis from Nucellar Tissues of Mango (Mangifera indica Linn.)  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 175-178

Callus induction and somatic embryogenesis were obtained from de-embryonated nucellar tissues of young mango (Mangifera indica Linn.) cultivar �Natumanga� fruits on MS medium supplemented with 4.52 �M 2,4-D. Mostly globular/heart shape...

Effect of Azospirillum on In Vitro Regenerated Bananas  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 171-174

Considering the current importance attributed to organic products, the present study was designed to evaluate the effect of Azospirillum, NPK fertilizer and their combinations on six categories of plants in bananas namely, nendran and poovan shoot ti...

Callogenesis of Sida rhombifolia L.: A Threatened Medicinal Plant  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 167-170

Protocol for callogenesis of a species belonging to the genus Sida (Malvaceae) has been standardized. Sida rhombifolia L. var. rhomboidea (Roxb.) Mast., the 'swetberela', is an important medicinal plant used in Rural Bengal. The plant is presently no...

High Frequency Plant Regeneration from Shoot Tip Explants of Chilli (Capsicum annuum)  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 163-166

Rice is the major food crop of more than half of the world population, and with the growing population, the demand on the improvement of this cereal crop has also been increase. The conventional breeding techniques which have been used have the limit...

In Vitro Propagation of Rhinacanthus nasutus: A Medicinal Herb  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 159-162

Rhinacanthus nasutus is an important medicinal plant which posses anticancer, anti fungal, antiviral properties. A regeneration protocol has been developed for Rhinacanthus nasutus using leaf and shoot tip nodal explants. Calli were induced on MS med...

An Efficient Protocol for Agrobacterium-mediated Genetic Transformation in Rubber Tree (Hevea brasiliensis)  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 155-158

We report high frequency genetic transformation in Hevea brasiliensis (Hb) using anther derived embryogenic calli as the target tissue which is one of the important factors to yield maximum transformation events. Agrobacterium-mediated genetic transf...

Molecular Characterization Of Cocoa (Theobroma cocoa L.) Germplasm Using RAPD Markers  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 151-154

Forty Cocoa (Theobroma cacao L.) accessions obtained from Nigeria were characterized using 12 random oligonucleotide primers by RAPD analysis. The Jaccard�s similarity coefficient estimated from 139 RAPD markers obtained using 12 random primers....

In Vitro Adventitious Rooting of Basilicum polystachyon Microshoots  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 135-144

This study was conducted with an aim to increase the rooting efficiency of in vitro regenerated microshoots of Basilicum polystachyon (L) Moench, an important traditional medicinal plant for successful transfer to field conditions. Microshoots regene...

Direct Shoot Regeneration from Mature Scutellum Explants of Sorghum bicolor L.  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 129-134

An effecient protocol for direct shoot regeneration from mature scutellum explants of Sorghum bicolor L. has been established using thidiazuron (TDZ). Pre-treatment of sorghum seeds on Murashige and Skoog (MS) medium with 1.0 mg/l TDZ for 10 days pla...

Rapid and Efficient Plant Regeneration in Emblica officinalis via Somatic Embryogenesis  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 123-128

Somatic embryogenesis was achieved in �Chakaiya�, a highly prized cultivar of Emblica officinalis. Zygotic embryos were cultured on Murashige and Skoog medium supplemented with varying concentrations of auxins and cytokinins. Murashige and ...

Mass Clonal Propagation through Shoot Tip Culture of Male Budless Banana  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 117-122

The possibility of clonal propagation of banana through tissue culture was explored. Rapidly multiplying cultures of dessert banana clones (cv. Binathuri) were established from shoot tips on a MS medium supplemented with various concentrations and co...

Hemagglutinin Neuraminidase (Hn) Protein of Peste Des Petits Ruminants Virus Expressed in Transgenic Tobacco is Biologically Active  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 109-116

Pestis des Petits ruminants virus (PPRV) is a Morbillivirus closely related to Rinderpest virus and measles virus and infects sheep and goats with high mortality rate and morbidity rates. The two surface glycoproteins of PPRV Hemagglutinin-neuraminid...

Plant Regeneration from Mature Embryo Derived Calli of Triticum aestivum and Triticum durum  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 103-108

Mature embryos from 16 genotypes of Triticum aestivum and 4 genotypes of T. durum were cultured on MS medium with 4 modifications viz. MS2D (2.0 mg.1-1 2,4-D), MS 5D (5.0 mg.1-1 2,4-D), MSDC (5.0 mg.1-1 2,4-D + 100 ml.l-1 coconut milk) and MSDK (2.0 ...

Effect of Jasmonic Acid on Growth Characteristics of Calli derived from Mature Embryos of Wheat Cultivars showing Differential Resistance to Karnal Bunt (Tilletia indica)  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 3-4 2006, Pages: 97-102

Effect of jasmonic acid (JA) was observed on the modulation of plant growth, differentiation and senescence under in vitro conditions. Calli raised from both Karnal bunt (KB) susceptible and resistant cultivars of wheat, were sub-cultured in MS mediu...

Genetic Stability of In Vitro raised Sugarcane Plantlets by RAPD Markers  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 1-2 2006, Pages: 93-96

Micropropagated plantlets produced by shoot-tip culture of two varieties of sugarcane were evaluated for genetic stability using randomly amplified polymorphic DNA (RAPD) analysis. Distinct and reproducible RAPD markers obtained through eight decamer...

Effect of Growth Regulators on Alkaloid Production from Adhatoda vasica and Adhatoda beddomei In Vitro  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 1-2 2006, Pages: 89-92

The alkaloids present in Adhatoda species namely vasicine and vasicinone are responsible for its pharmacological property. But the production of alkaloids is too low; hence in vitro strategies have been adopted to enhance the production of alkaloids....