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Agrobacterium-mediated Genetic Transformation of Groundnut (Arachis hypogaea L.) using Epicotyl Explants  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 1-2 2006, Pages: 73-76

Transformation of groundnut (Arachis hypogaea L.) was achieved using Agrobacterium tumefaciens strain LBA4404 harboring binary vectors pHS102 (containing uidA, nptII and Cry1Ac genes) pBI121 (containing uidA (GUS) and nptII genes) pBinAR (possesses a...

In Vitro Selection of Pigeon Pea Cell Lines and Regeneration of Plantlets from Tolerant Callus to Culture Filtrate of Fusarium odum Buttler  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 1-2 2006, Pages: 69-72

Fusarium odum causes severe wilt disease in Pigeon Pea (Cajanus cajan L.) Mill. The present study reports selection of calli tolerant to culture filtrate of Fusarium odum. Cotyledon calli were selected for their tolerance of F. odum culture filtrate ...

In Vitro Shoot Regeneration from Leaf Segments of Carnation (Dianthus caryophyllus L.) Via Indirect Organogenesis  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 1-2 2006, Pages: 65-68

Leaf segments were prepared and cultured on MS medium supplemented with different concentrations and combinations of growth regulators. Among the combinations tried, highest frequency of callus induction was obtained with 1.0 mg/l 2,4-D + 2.2 mg/l TD...

Shoot Induction from Megagametophyte of Cycas circinalis L.  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 1-2 2006, Pages: 59-64

Cycas circinalis is one of the surviving remnants of an ancient line of plants of the early Mesozoic period. None of the in vitro studies has been successful in establishing a functional protocol for artificial propagation of cycads. The objective of...

In Vitro Morphogenesis of Rauwolfia serpentina L. Through Cotyledons  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 1-2 2006, Pages: 53-58

Immature cotyledons of Rauwolfia serpentina were cultured on MS medium containing different concentrations and combinations of growth regulators. Analysis of variance exhibited highly significant differences among culture media combinations for callu...

In Vitro Propropagation of Cymbidium lowianum Rchb.F using Immature Seeds: A Floriculturally Important Orchid  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 1-2 2006, Pages: 47-52

Cymbidium lowianum Rchb.f., a floriculturally important orchid was cultured in vitro on Mitra (M) and Murashige and Skoog (MS) medium using immature seeds derived from mature capsules. Plant growth regulators such as BAP (6- benzylaminopurine), NAA (...

Possible Role of Receptor Like Kinase(S) in Karnal Bunt Pathogenesis and Resistance  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 1-2 2006, Pages: 41-46

Four sets of primers were selected to amplify the members of receptor-like kinase gene family. However, amplification was observed with only one set of primers and only in six out of 20 wheat genotypes tested. The set of primers that amplified 6 vari...

Biosynthesis of Capsaicin in Callus Cultures derived from Fruit Explants of Capsicum annuum L.  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 1-2 2006, Pages: 35-40

Biosynthesis of capsaicin (8-methyl-N-vanillyl-6-noneamide) was compared in callus cultures obtained from different explants (placenta and pericarp) of Capsicum annuum L var. HC-44 and Pusajwala fruits. MS medium supplemented with 2,4- D (2mg l-1) an...

Cytokinin Induces New Features of Sphagnum Gametophyte Development  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 1-2 2006, Pages: 29-34

Peat mosses (Sphagnaceae) are ideal model organisms to explore the limits of morphological adaptation due to both their relative phylogenetic isolation and the reproducibility of their thallus growth in vitro. In the present study, we have systematic...

Direct Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Ocimum sanctum L. (Tulsi): A Medicinal Herb  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 1-2 2006, Pages: 23-28

In vitro multiple shoot regeneration of Holy basil (Ocimum sanctum L.), an Indian medicinal herb has been accomplished on MS medium utilizing shoot tip and nodal explants. Direct multiple shoots differentiated within 6 weeks when explants were cultur...

In Vitro Flowering of Basilicum polystachyon (L) Moench  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 1-2 2006, Pages: 17-22

Multiple shoot regeneration from shoot tip explants of Basilicum polystachyon (L) Moench, was achieved on Murashige and Skoog's (MS) basal medium supplemented with 0.5 ?M naphthaleneacetic acid (NAA) and 2.5 ?M 6-benzyladenine (BA). Regenerated shoot...

Influence of Phytohormones on Growth and Production of Ajmalicine by Multiple Shoot Cultures of Catharanthus roseus  ...

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 1-2 2006, Pages: 11-16

Multiple shoots were induced from shoot tip explants of Catharanthus roseus and growth rate as well as ajmalicine production were also studied. Shoot cultures were developed on MS medium supplemented with high concentrations of cytokinins BAP (8.90 ?...

Biotechnological and Molecular Approaches for Improvement of Orchids  

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 7, Issue: 1-2 2006, Pages: 1-10

Orchids due to their extremely beautiful fascinating flowers occupy a significant position in the international floricultural trade of cut flower and pot plants. India, especially the north-eastern region of the country harboring 650 orchid species i...

In Vitro Adventitious Shoot Formation from Leaf Explants of Withania somnifera Dunal

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 3-4 2005, Pages: 163-166

An efficient protocol was developed for high frequency plant regeneration from leaf explants of Withania somnifera Dunal on Murashige and Skoog (1962) medium supplemented with different concentrations of Auxins and cytokinins. Frequency of shoot bud ...

Micropropagation of Musa Accuminata Colla

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 3-4 2005, Pages: 159-162

A rapid and reproducible regeneration protocol has been established in Musa acuminate Colla. Shoot tip explants were subjected to vertical cut before inoculation. Murashige and Skoog medium supplemented with 8 mg/l BA or a combination of 0.1 mg/l NAA...

Somatic Embryogenesis and Plant Regeneration in Phyllanthus emblica Linn

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 3-4 2005, Pages: 155-158

Proliferating friable callus was induced from zygotic embryos of hybrid Phyllanthus emblica Linn., a tropical as well as highly medicinal tree plant, on MS medium containging 1mgl-1 2,4-D and 1mgl-1 KIN. This friable callus differentiated into normal...

In Vitro Proliferation of Hyptis suaveolens Poit: An Ethno-Medicinal Herb

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 3-4 2005, Pages: 151-154

In the present study, plant regeneration was achieved via multiple shoots in Hyptis suaveolens. The apical buds of the three days old seedlings could be stimulated to undergo multiple shoot formation on Murashige and Skoog s medium supplemented with ...

In Vitro Multiplication of Wedelia chinensis (Osbeck) Merr

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 3-4 2005, Pages: 147-150

Wedelia chinensis plant is closely related to Eclipta alba and it is very specific for viral hepatitis. In vitro shoot multiplication from nodal and shoot tip explants was achieved on MS media containing BAP, Kn, IBA and IAA. The BAP and IBA combinat...

In Vitro Regeneration of Vitex negundo L., A Medicinal Shrub

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 3-4 2005, Pages: 143-146

Vitex negundo commonly known as five- leaved chaste tree or Nirgundi is a deciduous shrub with thin grey bark. A successful protocol for in vitro regeneration of V. negundo from nodal explants on MS medium supplemented with different concentrations o...

RAPD-based Genetic Diversity analysis of Aromatic Rice (Oryza sativa L)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 6, Issue: 3-4 2005, Pages: 133-142

Genetic diversity of a total aromatic rice lines and two aromatic controls were assessed using the randomly amplified polymorphic DNA (RAPD) technique. Based on their history of collection and pedigree, the rice genotypes were grouped as traditional ...