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In Vitro Production of Virus Free Plants in Citrus reticulate

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 147-150

Virus free plantlets were successfully produced using meristem tip as explants. Meristem dome with 2 leaf primordial (Size 100-200u) was cultured in modified MS liquid media (M19) containing 4 mg l-1 Thamine, 0.01mg l-1 Riboflavin and 0.01 mg. l-1 GA...

Regeneration in Chenopodium album L.via Organogenesis and Somatic Embryogenesis

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 143-146

Mode of plant regeneration from callus was studied in Chenopodium album L. (2n=18; broad leafed cytotype), a semi cultivated crop rich in ?-carotene. Scanning electron microscopic study revealed both organogenesis and somatic embryogenesis from the s...

In Vitro Callus Induction and Plantlet Regeneration from Indian Cotton Cultivars

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 137-142

Efficient plant regeneration from callus cultures of Indian cotton cultivars is reported here. Ten Indian cotton cultivars namely NCS3, NA920, NHH390, NA1325, PMC, LRA, MCU5, DCH32, Srisailam and Anjali were used for the present experiment. Different...

In Vitro Plant Regeneration from Leaf Sheath Cultures of Sugarcane via Organogenesis

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 131-136

In vitro morphogenesis was obtained from leaf sheath derived calli of three local varieties ( Isd-16, Isd-28 and Amrita ) of sugarcane (Saccharum officinarum L). The calli induced in 2,4-D (0.5mg./l) containing MS medium showed better regenerating ca...

Agrobacterium-mediated Genetic Transformation of Chickpea (Cicer arietinum L.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 125-130

Transformation of chickpea (Cicer arietinum L.) was achieved using Agrobacterium tumefaciens strain EHA105 harboring binary vector p35SGUSINT containing uidA (GUS) and npt II genes, and pJB90GI containing uid A (GUS) and hpt genes. A strong GUS expre...

Microsatellite Markers for Yellow Stem Borer (Scirpophaga incertulas Walker.) Resistance in Rice

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 117-124

The availability of molecular markers for various traits is a prerequisite for Marker Assisted Selection, Map based cloning etc. The possibility to tag genes for yellow stem borer resistance with microsatellite markers was investigated in the present...

In Vitro Plant Regeneration from Shoot Tip Explants of Panicum sumatrence

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 111-116

In vitro raised shoot tip explants from little millet were used to produce multiple shoots on a medium containing various concentrations of growth regulators. Among the combinations used, MS medium containing BAP (0.3 mg/l) and KIN (0.2 mg/l) combina...

Biotechnology of Sesame-An Oil Seed Crop

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 101-110

Sesamum (Sesamum indicum) is an ancient oil seed crop and grown in India from antiquity. The primary aim of sesame breeding is to improve yield as well as oil content. But, both biotic and abiotic stress factors are hampering the realization of the f...

Somatic Cell Genetics in Improvement of Tree Species

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 3-4 2002, Pages: 81-100

Nucellar Embryogenesis and Artificial Seed Production in Citrus reticulata.

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 1-2 2002, Pages: 77-80

A study was undertaken to develop a protocol for nucellar embryogenesis and artificial seed preparation in Khasimandarin. Nucellar embryoids were formed in 35 days after culturing in NE4 media containing MT basal with malt extract (500mg l-1) (W/V)BA...

Micropropagation of Tylophora asthmatica and Uraria picta

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 1-2 2002, Pages: 73-76

Tylophara asthmatica and Uraria picta are medicinal plants finding a wide range of applications in the Ayurvedic system of medicine. Nodal explants of these two plants were cultured on MS medium supplemented with BAP for shoot bud induction. The maxi...

Effect of certain Chemicals and Sucrose on Enhancement of In Vitro Bulblet Size of Lilium Sp. (Asiatic hybrid) Cv. Toscana

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 1-2 2002, Pages: 69-72

The in vitro bulblet growth of Lilium sp. (Asiatic hybrid) cv. Toscana was increased noticeably by supplementing the culture medium with maleic hydrazide, salicylic acid, paclobutrazol, and sucrose. Sucrose and maleic hydrazide enhanced the size of t...

Production of Plumbagin from Hairy Root Cultures of Plumbago rosea L.

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 1-2 2002, Pages: 65-68

Hairy root cultures were induced from an endangered plant Plumbago rosea after infecting the in vitro grown shoots with Agrobacterium rhizogenes and accumulation of plumbagin (2-methyl-5-hydroxy-1,4-naphthoquinone) was detected. Different media and m...

Somatic Embryogenesis in Indian Spinach (Beta palonga)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 1-2 2002, Pages: 59-64

A system for somatic embryogenesis and plant regeneration of Indian spinach from embryogenic callus line was established. Nodular compact embryogenic callus derived from nodal explants of eight day old seedlings was induced on MS basal medium supplem...

Somatic Embryogenesis and Agrobacterium-mediated Transformation In Chickpea (Cicer arietinum L.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 1-2 2002, Pages: 51-58

Agrobacterium-mediated transformation of Cicer arietinum L. was achieved from ICCV-10 and Annigeri varieties via somatic embryogenesis using mature and immature cotyledons, on MS medium fortified with B5 vitamins, different concentration of 2,4-D, 2,...

Transient Gene Expression in Two Pine Species by Microprojectile Bombardment

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 1-2 2002, Pages: 43-50

A biolistic particle delivery system was used to transform the uidA reporter gene driven by a maize polybiquitin promoter to cotyledons of P. roxburghii and embryos of P. contorta. Successful gene delivery was demonstrated by transient GUS expression...

In Vitro Plant Regeneration in Cotton by Multiple Shoot Induction

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 1-2 2002, Pages: 35-42

A rapid and simple multiple shoot induction protocol has been developed from cotyledonary node explant. The cotyledonary node explant consists of lower 0.5 mm portion of hypocotyl and cotyledonary node without any cotyledons attached. Murashige and S...

In Vitro Propagation of Drosera indica L. An Endangered Insectivorous Plant

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 1-2 2002, Pages: 29-34

Drosera indica L. is a threatened and endangered insectivorous plant. While there are successful tissue-culture protocols for a number of Drosera species, this is the first successful protocol for D. indica L. using stem segments as explants. Multipl...

Isozyme and RAPD Analysis of Cassava Germplasm : Identification of Duplicates in Exotic Collection

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 1-2 2002, Pages: 21-28

Esterase isozyme polymorphism was used to study duplicate accessions in cassava germplasm. 44 sets of morphological duplicates were analysed for variability at isozyme level. 34 different bands were obtained. 28 sets were found to be duplicates based...

Protoplast Isolation and Culture in Sugar Beet (Beta vulgaris L.)

PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume: 3, Issue: 1-2 2002, Pages: 11-20

In this study, attempts were made to optimize conditions for protoplast isolation from suspension and mesophyll cells, growth of isolated protoplasts and formation of callus from the cultured protoplasts. The enzyme mixture containing 1.0% hemicellul...

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